Supplementary data for "Copy number variation and selection during reprogramming to pluripotency"

  • Samer Hussein (Creator)
  • M. Sourour (Creator)
  • R. Hämäläinen (Creator)
  • Andras Nagy (Creator)
  • Sanna Vuoristo (Creator)
  • C. Olsson (Creator)
  • Karolina Lundin (Creator)
  • M. Mikkola (Creator)
  • Ras Trokovic (Creator)
  • Timo Otonkoski (Creator)
  • Nizar N. Batada (Creator)
  • Siemon Ng (Creator)
  • R. W. Ching (Creator)
  • D. P. Bazett-Jones (Creator)
  • Reija Autio (Creator)
  • Elisa Närvä (Creator)
  • Riitta Lahesmaa (Creator)
  • M. Peitz (Creator)
  • O. Brüstle (Creator)
  • K. Alitalo (Creator)



    The mechanisms underlying the low efficiency of reprogramming somatic cells into induced pluripotent stem (iPS) cells are poorly understood. There is a clear need to study whether the reprogramming process itself compromises genomic integrity and, through this, the efficiency of iPS cell establishment. Using a high-resolution single nucleotide polymorphism array, we compared copy number variations (CNVs) of different passages of human iPS cells with their fibroblast cell origins and with human embryonic stem (ES) cells. Here we show that significantly more CNVs are present in early-passage human iPS cells than intermediate passage human iPS cells, fibroblasts or human ES cells. Most CNVs are formed de novo and generate genetic mosaicism in early-passage human iPS cells. Most of these novel CNVs rendered the affected cells at a selective disadvantage. Remarkably, expansion of human iPS cells in culture selects rapidly against mutated cells, driving the lines towards a genetic state resembling human ES cells.
    Date made available2017
    PublisherTampere University of Technology
    • Copy number variation and selection during reprogramming to pluripotency

      Hussein, S. M., Batada, N. N., Vuoristo, S., Ching, R. W., Autio, R., Narva, E., Ng, S., Sourour, M., Hämäläinen, R., Olsson, C., Lundin, K., Mikkola, M., Trokovic, R., Peitz, M., Bruestle, O., Bazett-Jones, D. P., Alitalo, K., Lahesmaa, R., Nagy, A. & Otonkoski, T., 2011, In: Nature. 471, 7336, p. 58-62

      Research output: Contribution to journalArticleScientificpeer-review

      708 Citations (Scopus)

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