Optical 3D-Imaging Microscopy

The facility offers real 3D imaging of transparent materials and tissues in micrometer resolution. In addition to both bright-field and fluorescence microscopes, the facility has state-of-the-art data processing and analysis workstation available for visualization and 3D-image quantification.

Introduction
Optical 3D-Imaging Microscopy facility offers real 3D-imaging of transparent materials and tissues in micrometer resolution. It is situated at Computational Biophysics and Imaging Group (CBIG) in BioMediTech Arvo 2 building. It offers two different optical 3D-imaging systems: a Selective Plane Illumination Microscope (SPIM) and an Optical Projection Tomography (OPT) microscopes.

The systems are being designed and used for imaging transparent biomaterials such as hydrogels with or without cells but usual applications include visualization of anatomy (phenotyping), gene expression (in situ hybridization), protein distribution (immunohistochemistry or GFP expression), transgenic visualization (LacZ).

Typical specimens include mouse and chicken embryos, mouse/rat tissue and organs, zebrafish, drosophila, plants etc.

In addition to the imaging systems the facility has state-of-the-art data processing and analysis workstation available for visualization and 3D-image quantification.

ACKNOWLEDGEMENT
Many of the technologies are still experimental and in development. Thus, prior to publishing, please agree the level of acknowledgement/participation of the paper with the core contact persons.