A fast stimulability screening protocol for neuronal cultures on microelectrode arrays

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    Abstract

    Microelectrode arrays (MEAs) are used to study the electrical activity in brain slices and neuronal cultures. MEA experiments for the analysis of electrical stimulation responses require the tissue or culture to be prone to stimulation. For brain slices, potential stimulation sites may be directly visible in microscope, in which case the determination of stimulability at those locations is sufficient. In unstructured neuronal cultures, potential stimulation sites may not be known a priori, and spatial stimulability screening should be performed. Considering, e.g., 59 microelectrode sites, each to be stimulated several times, may result in long screening times, unacceptable with a MEA system without an integrated CO2 incubator, or in high stimulation effects on the networks. Here, we describe an implementation of a fast stimulation protocol employing pseudorandom stimulation site switching aiming at alleviating the network effects of the stimulability screening. In this paper, we show the usability of the proposed protocol by first detecting stimulable locations and subsequently apply repeated stimulation on the identified potentially stimulable locations to observe an exemplary neuronal pathway.
    Original languageEnglish
    Title of host publication2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
    Pages3440-3443
    Number of pages4
    DOIs
    Publication statusPublished - 2015
    Publication typeA4 Article in conference proceedings
    EventAnnual International Conference of the IEEE Engineering in Medicine and Biology Society -
    Duration: 1 Jan 1900 → …

    Conference

    ConferenceAnnual International Conference of the IEEE Engineering in Medicine and Biology Society
    Period1/01/00 → …

    Keywords

    • Biological neural networks
    • Delays
    • Electric potential
    • Electrical stimulation
    • Microelectrodes
    • Protocols

    Publication forum classification

    • Publication forum level 1

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