A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study

Veera Hautanen, Tarja Toimela, Martin Paparella, Tuula Heinonen

    Research output: Contribution to journalArticleScientificpeer-review

    1 Citation (Scopus)
    5 Downloads (Pure)

    Abstract

    The induction of vasculature formation is proposed to be a significant mechanism behind the non-genotoxic carcinogenicity of a chemical. The vasculature formation model used in this study is based on the coculture of human primary HUVECs and hASCs. This model was used to develop an assay to assess the induction of vasculature formation. Three assay protocols, based on different conditions, were developed and compared in order to identify the optimal conditions required. Some serum supplements and growth factors were observed to be essential for initiating vasculature formation. Of the studied putative positive reference chemicals, aspartame, sodium nitrite, bisphenol A and nicotine treatment led to a clear induction of vasculature formation, but arsenic and cadmium treatment only led to a slight increase. This human cell-based assay has the potential to be used as one test within a next generation testing battery, to assess the non-genotoxic carcinogenicity of a chemical through the mechanism of vasculature formation induction.

    Original languageEnglish
    Pages (from-to)188-203
    Number of pages16
    JournalAlternatives to Laboratory Animals
    Volume51
    Issue number3
    Early online date2023
    DOIs
    Publication statusPublished - May 2023
    Publication typeA1 Journal article-refereed

    Keywords

    • angiogenesis
    • in vitro model
    • non-genotoxic carcinogenicity
    • vascular model
    • vascular toxicity
    • vasculature formation
    • vasculogenesis

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • General Medicine

    Fingerprint

    Dive into the research topics of 'A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study'. Together they form a unique fingerprint.

    Cite this