A panel of bioluminescent whole-cell bacterial biosensors for the screening for new antibacterial substances from natural extracts

Emmi Poikulainen, Jenni Tienaho, Tytti Sarjala, Ville Santala

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Whole-cell bacterial biosensors can be applied for the screening of antibacterial properties of extracts. We constructed a biosensor panel consisting of four different bacterial biosensor strains: Escherichia, Staphylococcus, Acinetobacter and Pseudomonas for expanded screening potential. The functionality of the panel was first evaluated with known antibacterial compounds: ethanol, naphthoquinones (juglone, lawsone, plumbagin) and a flavonoid (quercetin). Natural extracts comprise a vast source of potential new antibacterials for diverse functional purposes. To demonstrate the utilization of the panel for screening of a demanding sample material, round-leaved sundew (Drosera rotundifolia) extracts were used as an example. Differences between field- and laboratory originating sundew extracts could be detected. This demonstrates the efficiency of the developed biosensor panel in the rapid screening of the antibacterial properties of plant extracts.

Original languageEnglish
Article number106083
Number of pages5
JournalJournal of Microbiological Methods
Volume178
DOIs
Publication statusPublished - Nov 2020
Publication typeA1 Journal article-refereed

Keywords

  • Antibacterial
  • Bioluminescence
  • Biosensor cells
  • Drosera rotundifolia
  • High-throughput screening
  • Natural extracts

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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