TY - JOUR
T1 - A reverse vaccinology approach on transmembrane carbonic anhydrases from Plasmodium species as vaccine candidates for malaria prevention
AU - Zolfaghari Emameh, Reza
AU - Barker, Harlan R.
AU - Turpeinen, Hannu
AU - Parkkila, Seppo
AU - Hytönen, Vesa P.
N1 - Funding Information:
The authors acknowledge the financial supports from the National Institute of Genetic Engineering and Biotechnology (NIGEB), Academy of Finland, Finnish Cultural Foundation (Pirkanmaa Regional Fund), Sigrid Juselius Foundation, Jane and Aatos Erkko Foundation, and Tampere University Hospital.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/6
Y1 - 2022/6
N2 - Background: Malaria is a significant parasitic infection, and human infection is mediated by mosquito (Anopheles) biting and subsequent transmission of protozoa (Plasmodium) to the blood. Carbonic anhydrases (CAs) are known to be highly expressed in the midgut and ectoperitrophic space of Anopheles gambiae. Transmembrane CAs (tmCAs) in Plasmodium may be potential vaccine candidates for the control and prevention of malaria. Methods: In this study, two groups of transmembrane CAs, including α-CAs and one group of η-CAs were analysed by immunoinformatics and computational biology methods, such as predictions on transmembrane localization of CAs from Plasmodium spp., affinity and stability of different HLA classes, antigenicity of tmCA peptides, epitope and proteasomal cleavage of Plasmodium tmCAs, accessibility of Plasmodium tmCAs MHC-ligands, allergenicity of Plasmodium tmCAs, disulfide-bond of Plasmodium tmCAs, B cell epitopes of Plasmodium tmCAs, and Cell type-specific expression of Plasmodium CAs. Results: Two groups of α-CAs and one group of η-CAs in Plasmodium spp. were identified to contain tmCA sequences, having high affinity towards MHCs, high stability, and strong antigenicity. All putative tmCAs were predicted to contain sequences for proteasomal cleavage in antigen presenting cells (APCs). Conclusions: The predicted results revealed that tmCAs from Plasmodium spp. can be potential targets for vaccination against malaria.
AB - Background: Malaria is a significant parasitic infection, and human infection is mediated by mosquito (Anopheles) biting and subsequent transmission of protozoa (Plasmodium) to the blood. Carbonic anhydrases (CAs) are known to be highly expressed in the midgut and ectoperitrophic space of Anopheles gambiae. Transmembrane CAs (tmCAs) in Plasmodium may be potential vaccine candidates for the control and prevention of malaria. Methods: In this study, two groups of transmembrane CAs, including α-CAs and one group of η-CAs were analysed by immunoinformatics and computational biology methods, such as predictions on transmembrane localization of CAs from Plasmodium spp., affinity and stability of different HLA classes, antigenicity of tmCA peptides, epitope and proteasomal cleavage of Plasmodium tmCAs, accessibility of Plasmodium tmCAs MHC-ligands, allergenicity of Plasmodium tmCAs, disulfide-bond of Plasmodium tmCAs, B cell epitopes of Plasmodium tmCAs, and Cell type-specific expression of Plasmodium CAs. Results: Two groups of α-CAs and one group of η-CAs in Plasmodium spp. were identified to contain tmCA sequences, having high affinity towards MHCs, high stability, and strong antigenicity. All putative tmCAs were predicted to contain sequences for proteasomal cleavage in antigen presenting cells (APCs). Conclusions: The predicted results revealed that tmCAs from Plasmodium spp. can be potential targets for vaccination against malaria.
KW - Carbonic anhydrase
KW - Immunoinformatics
KW - Malaria
KW - Plasmodium spp
KW - Reverse vaccinology
KW - Vaccine
U2 - 10.1186/s12936-022-04186-7
DO - 10.1186/s12936-022-04186-7
M3 - Article
C2 - 35706028
AN - SCOPUS:85131962090
SN - 1475-2875
VL - 21
JO - Malaria Journal
JF - Malaria Journal
M1 - 189
ER -
