Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics

Miao Yan; Ioannis A. Fotidis; Hailin Tian; Benyamin Khoshnevisan; Laura Treu; Panagiotis Tsapekos; Irini Angelidaki

doi:10.1016/j.biortech.2019.121376

Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics

Miao Yan
, Ioannis A. Fotidis^*
, Hailin Tian
, Benyamin Khoshnevisan
, Laura Treu
, Panagiotis Tsapekos
, Irini Angelidaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

115 Citations (Scopus)

Abstract

The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH₄⁺-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.

Original language	English
Article number	121376
Journal	Bioresource Technology
Volume	286
DOIs	https://doi.org/10.1016/j.biortech.2019.121376
Publication status	Published - Aug 2019
Externally published	Yes
Publication type	A1 Journal article-refereed

Funding

This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH3-Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327). We acknowledge financial support from MUDP project “VARGA - VAnd Ressource Genvindings Anlægget”. Miao Yan would like to thank for the financial support from China Scholarship Council .

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy
SDG 11 Sustainable Cities and Communities
SDG 12 Responsible Consumption and Production

Keywords

Aceticlastic pathway
Ammonia acclimatization
Hydrogenotrophic pathway
Metabolic pathway shift
Methanosarcina soligelidi

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2019.121376

Cite this

@article{b41f252faae845a39006d51a0ced91be,

title = "Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics",

abstract = "The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10\%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.",

keywords = "Aceticlastic pathway, Ammonia acclimatization, Hydrogenotrophic pathway, Metabolic pathway shift, Methanosarcina soligelidi",

author = "Miao Yan and Fotidis, \{Ioannis A.\} and Hailin Tian and Benyamin Khoshnevisan and Laura Treu and Panagiotis Tsapekos and Irini Angelidaki",

note = "Funding Information: This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH3-Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327). We acknowledge financial support from MUDP project “VARGA - VAnd Ressource Genvindings Anl{\ae}gget”. Miao Yan would like to thank for the financial support from China Scholarship Council . Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2019",

month = aug,

doi = "10.1016/j.biortech.2019.121376",

language = "English",

volume = "286",

journal = "Bioresource Technology",

issn = "0960-8524",

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T2 - Focusing on microbial community dynamics

AU - Yan, Miao

AU - Fotidis, Ioannis A.

AU - Tian, Hailin

AU - Khoshnevisan, Benyamin

AU - Treu, Laura

AU - Tsapekos, Panagiotis

AU - Angelidaki, Irini

N1 - Funding Information: This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH3-Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327). We acknowledge financial support from MUDP project “VARGA - VAnd Ressource Genvindings Anlægget”. Miao Yan would like to thank for the financial support from China Scholarship Council . Publisher Copyright: © 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019/8

Y1 - 2019/8

N2 - The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.

AB - The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.

KW - Aceticlastic pathway

KW - Ammonia acclimatization

KW - Hydrogenotrophic pathway

KW - Metabolic pathway shift

KW - Methanosarcina soligelidi

U2 - 10.1016/j.biortech.2019.121376

DO - 10.1016/j.biortech.2019.121376

M3 - Article

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SN - 0960-8524

VL - 286

JO - Bioresource Technology

JF - Bioresource Technology

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ER -

Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

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