TY - JOUR
T1 - Active MMP-8 point-of-care (PoC)/chairside enzyme-test as an adjunctive tool for early and real-time diagnosis of peri-implantitis
AU - Lähteenmäki, Hanna
AU - Tervahartiala, Taina
AU - Räisänen, Ismo T.
AU - Pärnänen, Pirjo
AU - Mauramo, Matti
AU - Gupta, Shipra
AU - Sampson, Victoria
AU - Rathnayake, Nilminie
AU - Heikkinen, Anna Maria
AU - Alassiri, Saeed
AU - Gieselmann, Dirk Rolf
AU - Frankenberger, Roland
AU - Sorsa, Timo
N1 - Funding Information:
This study was funded by the Helsinki and Uusimaa Hospital District (HUS) (grant numbers TYH2016251, TYH2017251, TYH2018229, TYH2019319, Y1014SL017, Y1014SL018, Y1014SULE1), Finland; Finnish Dental Association Apollonia, Finland; Karolinska Institutet, Sweden; P.P. has received a grant from the Minerva Foundation, Selma and Maja‐Lisa Selander Foundation, The Finnish Dental Society Apollonia dissertation grant and Faculty of Biosciences (University of Helsinki) dissertation completion grant; and ITR has received dissertation grants from The Yrjö Jahnsson Foundation sr, The Paulo Foundation, The Emil Aaltonen Foundation sr, The Juhani Aho Foundation for Medical Research sr, The Orion Research Foundation sr, The Finnish Dental Society Apollonia and Helsingin Seudun Hammaslääkärit r.y. (Dentists of Helsinki Region Association), Finland.
Funding Information:
This study was funded by the Helsinki and Uusimaa Hospital District (HUS) (grant numbers TYH2016251, TYH2017251, TYH2018229, TYH2019319, Y1014SL017, Y1014SL018, Y1014SULE1), Finland; Finnish Dental Association Apollonia, Finland; Karolinska Institutet, Sweden; P.P. has received a grant from the Minerva Foundation, Selma and Maja-Lisa Selander Foundation, The Finnish Dental Society Apollonia dissertation grant and Faculty of Biosciences (University of Helsinki) dissertation completion grant; and ITR has received dissertation grants from The Yrj? Jahnsson Foundation sr, The Paulo Foundation, The Emil Aaltonen Foundation sr, The Juhani Aho Foundation for Medical Research sr, The Orion Research Foundation sr, The Finnish Dental Society Apollonia and Helsingin Seudun Hammasl??k?rit r.y. (Dentists of Helsinki Region Association), Finland.
Publisher Copyright:
© 2022 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
PY - 2022/4
Y1 - 2022/4
N2 - Objective: The aim of this study was to investigate the utility of the active matrix metalloproteinase (aMMP-8)-point-of-care (PoC) test as a quantitative real-time chair-side diagnostic tool for peri-implant diagnosis, as well as assess the potentially developing and ongoing risk relative to the traditional clinical methods. Background: Current peri-implant and periodontal disease diagnoses rely on clinical and radiological examinations. This case-control study investigated the applicability of aMMP-8-PoC immunotest for quantitative real-time diagnosis and monitoring of dental implants in health and disease. Methods: Sixty-eight patients visiting a specialist clinic for maintenance following dental implant placement underwent assessment of their peri-implant health. aMMP-8-PoC peri-implant sulcular fluid (PISF) lateral-flow immunotests were performed using ImplantSafe® technology quantitated by ORALyzer®. In addition, the PISF samples were analyzed for total MMP-8, calprotectin, and interleukin (IL)-6 by enzyme-linked immunosorbent assays (ELISA), aMMP-8 by western immunoblot, and MMP-2 and MMP-9 by gelatin zymography. Results: The aMMP-8-PoC test promptly recorded and reflected peri-implant disease, differentiating it clearly from health. X-ray findings (bone loss > 2 mm), peri-implant pocket depth ≥ 3 mm, and bleeding on probing were significantly more prevalent among implants positive for the aMMP-8-PoC test. aMMP-8/ORALyzer analysis was more precise in recording disease than total MMP-8, calprotectin, IL-6, MMP-2, and MMP-9. Conclusions: The aMMP-8-PoC test can be conveniently implemented to alert for and detect active collagenolysis affecting peri-implant tissues, both in the early and advanced stages of the disease. Active and fragmented MMP-8 exhibits a strong and significant association with peri-implantitis as compared to total MMP-8 and other biomarkers and can be utilized as the POC/chairside biomarker of choice in the new classification of peri-implantitis.
AB - Objective: The aim of this study was to investigate the utility of the active matrix metalloproteinase (aMMP-8)-point-of-care (PoC) test as a quantitative real-time chair-side diagnostic tool for peri-implant diagnosis, as well as assess the potentially developing and ongoing risk relative to the traditional clinical methods. Background: Current peri-implant and periodontal disease diagnoses rely on clinical and radiological examinations. This case-control study investigated the applicability of aMMP-8-PoC immunotest for quantitative real-time diagnosis and monitoring of dental implants in health and disease. Methods: Sixty-eight patients visiting a specialist clinic for maintenance following dental implant placement underwent assessment of their peri-implant health. aMMP-8-PoC peri-implant sulcular fluid (PISF) lateral-flow immunotests were performed using ImplantSafe® technology quantitated by ORALyzer®. In addition, the PISF samples were analyzed for total MMP-8, calprotectin, and interleukin (IL)-6 by enzyme-linked immunosorbent assays (ELISA), aMMP-8 by western immunoblot, and MMP-2 and MMP-9 by gelatin zymography. Results: The aMMP-8-PoC test promptly recorded and reflected peri-implant disease, differentiating it clearly from health. X-ray findings (bone loss > 2 mm), peri-implant pocket depth ≥ 3 mm, and bleeding on probing were significantly more prevalent among implants positive for the aMMP-8-PoC test. aMMP-8/ORALyzer analysis was more precise in recording disease than total MMP-8, calprotectin, IL-6, MMP-2, and MMP-9. Conclusions: The aMMP-8-PoC test can be conveniently implemented to alert for and detect active collagenolysis affecting peri-implant tissues, both in the early and advanced stages of the disease. Active and fragmented MMP-8 exhibits a strong and significant association with peri-implantitis as compared to total MMP-8 and other biomarkers and can be utilized as the POC/chairside biomarker of choice in the new classification of peri-implantitis.
KW - biomarkers
KW - diagnosis
KW - matrix metalloproteinase 8
KW - peri-implantitis
KW - preventive medicine
U2 - 10.1002/cre2.537
DO - 10.1002/cre2.537
M3 - Article
C2 - 35118828
AN - SCOPUS:85124165898
SN - 2057-4347
VL - 8
SP - 485
EP - 496
JO - Clinical And Experimental Dental Research
JF - Clinical And Experimental Dental Research
IS - 2
ER -