Advancing chemical safety assessment through an omics-based characterization of the test system-chemical interaction

Giusy del Giudice, Giorgia Migliaccio, Nicoletta D’Alessandro, Laura Aliisa Saarimäki, Marcella Torres Maia, Maria Emilia Annala, Jenni Leppänen, Lena Möbus, Alisa Pavel, Maaret Vaani, Anna Vallius, Laura Ylä-Outinen, Dario Greco, Angela Serra

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Assessing chemical safety is essential to evaluate the potential risks of chemical exposure to human health and the environment. Traditional methods relying on animal testing are being replaced by 3R (reduction, refinement, and replacement) principle-based alternatives, mainly depending on in vitro test methods and the Adverse Outcome Pathway framework. However, these approaches often focus on the properties of the compound, missing the broader chemical-biological interaction perspective. Currently, the lack of comprehensive molecular characterization of the in vitro test system results in limited real-world representation and contextualization of the toxicological effect under study. Leveraging omics data strengthens the understanding of the responses of different biological systems, emphasizing holistic chemical-biological interactions when developing in vitro methods. Here, we discuss the relevance of meticulous test system characterization on two safety assessment relevant scenarios and how omics-based, data-driven approaches can improve the future generation of alternative methods.

Original languageEnglish
Article number1294780
Number of pages7
JournalFrontiers in Toxicology
Publication statusPublished - 2023
Publication typeA1 Journal article-refereed


  • AOP
  • chemical safety assessment
  • chemical-biological interaction
  • mechanism of action
  • nanosafety
  • new approach methodologies
  • test system characterization
  • toxicogenomics

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
  • Toxicology


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