Affinity-independent elution of antibody-displaying phages using cleavable DNA linker containing streptavidin beads

Ville Santala; Petri Saviranta

doi:10.1016/j.jim.2003.10.013

Affinity-independent elution of antibody-displaying phages using cleavable DNA linker containing streptavidin beads

Ville Santala, Petri Saviranta

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

Abstract

The search for high-affinity antibodies from phage libraries by panning is often complicated by inefficient detachment of the captured phage particles from the antigen-coated solid phase. General elution methods, independent on the bioaffinity interaction, would be desirable for high-throughput antibody screening. We have developed a bioaffinity-independent elution method based on the use of commercially available streptavidin paramagnetic particles.

Original language	English
Pages (from-to)	159-163
Number of pages	5
Journal	JOURNAL OF IMMUNOLOGICAL METHODS
Volume	284
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2003.10.013
Publication status	Published - Jan 2004
Externally published	Yes
Publication type	A1 Journal article-refereed

Keywords

Antibody fragment
High-throughput antibody screening
Paramagnetic streptavidin particles
Phage display

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jim.2003.10.013

Cite this

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title = "Affinity-independent elution of antibody-displaying phages using cleavable DNA linker containing streptavidin beads",

abstract = "The search for high-affinity antibodies from phage libraries by panning is often complicated by inefficient detachment of the captured phage particles from the antigen-coated solid phase. General elution methods, independent on the bioaffinity interaction, would be desirable for high-throughput antibody screening. We have developed a bioaffinity-independent elution method based on the use of commercially available streptavidin paramagnetic particles.",

keywords = "Antibody fragment, High-throughput antibody screening, Paramagnetic streptavidin particles, Phage display",

author = "Ville Santala and Petri Saviranta",

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AU - Santala, Ville

AU - Saviranta, Petri

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N2 - The search for high-affinity antibodies from phage libraries by panning is often complicated by inefficient detachment of the captured phage particles from the antigen-coated solid phase. General elution methods, independent on the bioaffinity interaction, would be desirable for high-throughput antibody screening. We have developed a bioaffinity-independent elution method based on the use of commercially available streptavidin paramagnetic particles.

AB - The search for high-affinity antibodies from phage libraries by panning is often complicated by inefficient detachment of the captured phage particles from the antigen-coated solid phase. General elution methods, independent on the bioaffinity interaction, would be desirable for high-throughput antibody screening. We have developed a bioaffinity-independent elution method based on the use of commercially available streptavidin paramagnetic particles.

KW - Antibody fragment

KW - High-throughput antibody screening

KW - Paramagnetic streptavidin particles

KW - Phage display

U2 - 10.1016/j.jim.2003.10.013

DO - 10.1016/j.jim.2003.10.013

M3 - Article

C2 - 14736426

AN - SCOPUS:0347003697

SN - 0022-1759

VL - 284

SP - 159

EP - 163

JO - JOURNAL OF IMMUNOLOGICAL METHODS

JF - JOURNAL OF IMMUNOLOGICAL METHODS

IS - 1-2

ER -

Affinity-independent elution of antibody-displaying phages using cleavable DNA linker containing streptavidin beads

Abstract

Keywords

ASJC Scopus subject areas

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