Analysis of ATP-Induced Ca2+ Responses at Single Cell Level in Retinal Pigment Epithelium Monolayers.

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

1 Citation (Scopus)


Calcium is one of the most important second messengers in cells and thus involved in a variety of physiological processes. In retinal pigment epithelium (RPE), Ca2+ and its ATP-dependent signaling pathways play important roles in the retina maintenance functions. Changes in intracellular Ca2+ concentration can be measured from living cells by Ca2+ imaging. Combining these measurements with quantitative analysis of Ca2+ response properties enables studies of signaling pathways affecting RPE functions. However, robust tools for response analysis from large cell populations are lacking. We developed MATLAB-based analysis tools for single cell level Ca2+ response data recorded from large fields of intact RPE monolayers. The analysis revealed significant heterogeneity in ATP-induced Ca2+ responses inside cell populations regarding magnitude and response kinetics. Further analysis including response grouping and parameter correlations allowed us to characterize the populations at the level of single cells.
Original languageEnglish
Title of host publicationRetinal Degenerative Diseases
EditorsCatherine Bowes Rickman
Number of pages6
ISBN (Print)978-3-030-27377-4
Publication statusPublished - 2019
Publication typeA4 Article in a conference publication
EventInternational Symposium on Retinal Degeneration -
Duration: 1 Jan 2019 → …

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598


ConferenceInternational Symposium on Retinal Degeneration
Period1/01/19 → …


  • ATP-induced Ca2+ response
  • Ca2+ imaging
  • Human embryonic stem cells
  • Image analysis
  • Retinal pigment epithelium

Publication forum classification

  • Publication forum level 1


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