Angiogenic Effect of Pravastatin Alone and with Sera from Healthy and Complicated Pregnancies Studied by in vitro Vasculogenesis/Angiogenesis Assay

Anita Virtanen, Outi Huttala, Kati Tihtonen, Tarja Toimela, Tuula Heinonen, Hannele Laivuori, Jukka Uotila

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Objective: To determine the direct effect of pravastatin on angiogenesis and to study the interaction between pravastatin and maternal sera from women with early- or late-onset pre-eclampsia (PE), intrauterine growth restriction, or healthy pregnancy. Methods: We collected 5 maternal serum samples from each group. The effect of pravastatin on angiogenesis was assessed with and without maternal sera by quantifying tubule formation in a human-based in vitro assay. Pravastatin was added at 20, 1,000, and 8,000 ng/mL concentrations. Concentrations of angiogenic and inflammatory biomarkers in serum and in test medium after supplementation of serum alone and with pravastatin (1,000 ng/mL) were measured. Results: Therapeutic concentration of pravastatin (20 ng/mL) did not have significant direct effect on angiogenesis, but the highest concentrations inhibited angiogenesis. Pravastatin did not change the levels of biomarkers in the test media. There were no changes in angiogenesis when therapeutic dose of pravastatin was added with maternal sera, but there was a trend to wide individual variation towards enhanced angiogenesis, particularly in the early-onset PE group. Conclusions: At therapeutic concentration, pravastatin alone or with maternal sera has no significant effect on angiogenesis, but at high concentrations the effect seems to be anti-angiogenic estimated by in vitro assay.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalJournal of Vascular Research
Volume58
Issue number3
DOIs
Publication statusPublished - May 2021
Publication typeA1 Journal article-refereed

Keywords

  • Angiogenic biomarkers
  • In vitro angiogenesis
  • Intrauterine growth restriction
  • Pravastatin
  • Pre-eclampsia

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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