Angiogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Nanofibrillated Cellulose Hydrogel

Elle Koivunotko, Jasmi Snirvi, Arto Merivaara, Riina Harjumäki, Swarna Rautiainen, Minna Kelloniemi, Kirsi Kuismanen, Susanna Miettinen, Marjo Yliperttula, Raili Koivuniemi

    Research output: Contribution to journalArticleScientificpeer-review

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    Adipose-derived mesenchymal stromal cells (ASCs) hold great potential for cellular therapies by having immunomodulatory behavior and tissue regenerative properties. Due to the capability of ASCs to differentiate into endothelial cells (ECs) and other angiogenic cell types, such as pericytes, ASCs are a highly valuable source for stimulating angiogenesis. However, cellular therapies in tissue engineering have faced challenges in poor survival of the cells after transplantation, which is why a protective biomaterial scaffold is required. In this work, we studied the potential of nanofibrillated cellulose (NFC) hydrogel to be utilized as a suitable matrix for three-dimensional (3D) cell culturing of human-derived ASCs (hASCs) and studied their angiogenic properties and differentiation potential in ECs and pericytes. In addition, we tested the effect of hASC-conditioned medium and stimulation with angiopoietin-1 (Ang-1) on human umbilical vein endothelial cells (HUVECs) to induce blood vessel-type tube formation in NFC hydrogel. The hASCs were successfully 3D cell cultured in NFC hydrogel as they formed spheroids and had high cell viability with angiogenic features. Most importantly, they showed angiogenic potential by having pericyte-like characteristics when differentiated in EC medium, and their conditioned medium improved HUVEC viability and tube formation, which recalls the active paracrine properties. This study recommends NFC hydrogel for future use as an animal-free biomaterial scaffold for hASCs in therapeutic angiogenesis and other cell therapy purposes.

    Original languageEnglish
    Article number2584
    Number of pages21
    Issue number10
    Publication statusPublished - Oct 2022
    Publication typeA1 Journal article-refereed


    • adipose-derived mesenchymal stromal cell
    • angiogenesis
    • endothelial differentiation
    • nanofibrillated cellulose hydrogel
    • pericyte

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • General Biochemistry,Genetics and Molecular Biology


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