Arginine combination with fluoride and calcium glycerophosphate: effects of concentration and on biofilm fluid

Marina Lins Miranda; Karina Borges Salomão; Alberto Carlos Botazzo Delbem; Marcelle Danelon; Elis Rodrigues Oliveira Barbosa; Caio Sampaio; Lucas Arrais Campos; Fernanda Lourenção Brighenti

doi:10.1080/17460913.2024.2411921

Arginine combination with fluoride and calcium glycerophosphate: effects of concentration and on biofilm fluid

Marina Lins Miranda
, Karina Borges Salomão
, Alberto Carlos Botazzo Delbem
, Marcelle Danelon
, Elis Rodrigues Oliveira Barbosa
, Caio Sampaio
, Lucas Arrais Campos
, Fernanda Lourenção Brighenti^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

2 Citations (Scopus)

Abstract

Aim: To study the influence of varying concentrations of arginine (Arg) combined with fluoride (F) and/or calcium glycerophosphate (CaGP) on biofilms. Materials & methods: Biofilms were analyzed for acidogenicity, microbial viability and Ca, F and inorganic phosphorus (P) concentrations. Results: For total bacteria, the lowest viability was found in F-containing groups, regardless of the arginine concentrations and presence of CaGP. For aciduric bacteria, no significant differences were found among arginine concentrations in the presence of F. For MS, arginine concentrations did not influence MS viability in the presence of fluoride and CaGP only decreased viability at 3.2% Arg concentration. The arginine-treated groups showed the lowest acidogenicity. For ion concentrations in biofilms, CaGP showed the highest values for P; Arg+F for F; and CaGP/Arg+CaGP for Ca. Conclusion: Different concentrations of arginine did not affect the microbial viability or acidogenicity of biofilms. Moreover, 0.8% Arg did not increase ion concentration in biofilm fluid.

Original language	English
Pages (from-to)	201-212
Journal	Future Microbiology
Volume	20
Issue number	3
Early online date	2024
DOIs	https://doi.org/10.1080/17460913.2024.2411921
Publication status	Published - 2025
Publication type	A1 Journal article-refereed

Funding

This work was supported by the Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior \u2013 Brasil (CAPES) \u2013 Finance Code 001, by the S\u00E3o Paulo Research Foundation (FAPESP) (grant 2019/08375-0 and 2020/16330-3) and by Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico\u2014CNPq [grant number 313473/2019-6].

Funders	Funder number
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Fundação de Amparo à Pesquisa do Estado de São Paulo	2019/08375-0, 2020/16330-3
Conselho Nacional de Desenvolvimento Científico e Tecnológico	313473/2019-6

Keywords

arginine
biofilms
calcium glycerophosphates
dental caries
fluorides

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

Access to Document

10.1080/17460913.2024.2411921

Cite this

@article{a732b84ac9cb4068a829125855f75952,

title = "Arginine combination with fluoride and calcium glycerophosphate: effects of concentration and on biofilm fluid",

abstract = "Aim: To study the influence of varying concentrations of arginine (Arg) combined with fluoride (F) and/or calcium glycerophosphate (CaGP) on biofilms. Materials \& methods: Biofilms were analyzed for acidogenicity, microbial viability and Ca, F and inorganic phosphorus (P) concentrations. Results: For total bacteria, the lowest viability was found in F-containing groups, regardless of the arginine concentrations and presence of CaGP. For aciduric bacteria, no significant differences were found among arginine concentrations in the presence of F. For MS, arginine concentrations did not influence MS viability in the presence of fluoride and CaGP only decreased viability at 3.2\% Arg concentration. The arginine-treated groups showed the lowest acidogenicity. For ion concentrations in biofilms, CaGP showed the highest values for P; Arg+F for F; and CaGP/Arg+CaGP for Ca. Conclusion: Different concentrations of arginine did not affect the microbial viability or acidogenicity of biofilms. Moreover, 0.8\% Arg did not increase ion concentration in biofilm fluid.",

keywords = "arginine, biofilms, calcium glycerophosphates, dental caries, fluorides",

author = "Miranda, \{Marina Lins\} and Salom{\~a}o, \{Karina Borges\} and \{Botazzo Delbem\}, \{Alberto Carlos\} and Marcelle Danelon and \{Oliveira Barbosa\}, \{Elis Rodrigues\} and Caio Sampaio and Campos, \{Lucas Arrais\} and Brighenti, \{Fernanda Louren{\c c}{\~a}o\}",

note = "Publisher Copyright: {\textcopyright} 2024 Informa UK Limited, trading as Taylor \& Francis Group.",

year = "2025",

doi = "10.1080/17460913.2024.2411921",

language = "English",

volume = "20",

pages = "201--212",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "American School of Classical Studies at Athens",

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TY - JOUR

T1 - Arginine combination with fluoride and calcium glycerophosphate

T2 - effects of concentration and on biofilm fluid

AU - Miranda, Marina Lins

AU - Salomão, Karina Borges

AU - Botazzo Delbem, Alberto Carlos

AU - Danelon, Marcelle

AU - Oliveira Barbosa, Elis Rodrigues

AU - Sampaio, Caio

AU - Campos, Lucas Arrais

AU - Brighenti, Fernanda Lourenção

PY - 2025

Y1 - 2025

N2 - Aim: To study the influence of varying concentrations of arginine (Arg) combined with fluoride (F) and/or calcium glycerophosphate (CaGP) on biofilms. Materials & methods: Biofilms were analyzed for acidogenicity, microbial viability and Ca, F and inorganic phosphorus (P) concentrations. Results: For total bacteria, the lowest viability was found in F-containing groups, regardless of the arginine concentrations and presence of CaGP. For aciduric bacteria, no significant differences were found among arginine concentrations in the presence of F. For MS, arginine concentrations did not influence MS viability in the presence of fluoride and CaGP only decreased viability at 3.2% Arg concentration. The arginine-treated groups showed the lowest acidogenicity. For ion concentrations in biofilms, CaGP showed the highest values for P; Arg+F for F; and CaGP/Arg+CaGP for Ca. Conclusion: Different concentrations of arginine did not affect the microbial viability or acidogenicity of biofilms. Moreover, 0.8% Arg did not increase ion concentration in biofilm fluid.

AB - Aim: To study the influence of varying concentrations of arginine (Arg) combined with fluoride (F) and/or calcium glycerophosphate (CaGP) on biofilms. Materials & methods: Biofilms were analyzed for acidogenicity, microbial viability and Ca, F and inorganic phosphorus (P) concentrations. Results: For total bacteria, the lowest viability was found in F-containing groups, regardless of the arginine concentrations and presence of CaGP. For aciduric bacteria, no significant differences were found among arginine concentrations in the presence of F. For MS, arginine concentrations did not influence MS viability in the presence of fluoride and CaGP only decreased viability at 3.2% Arg concentration. The arginine-treated groups showed the lowest acidogenicity. For ion concentrations in biofilms, CaGP showed the highest values for P; Arg+F for F; and CaGP/Arg+CaGP for Ca. Conclusion: Different concentrations of arginine did not affect the microbial viability or acidogenicity of biofilms. Moreover, 0.8% Arg did not increase ion concentration in biofilm fluid.

KW - arginine

KW - biofilms

KW - calcium glycerophosphates

KW - dental caries

KW - fluorides

U2 - 10.1080/17460913.2024.2411921

DO - 10.1080/17460913.2024.2411921

M3 - Article

AN - SCOPUS:85208496956

SN - 1746-0913

VL - 20

SP - 201

EP - 212

JO - Future Microbiology

JF - Future Microbiology

IS - 3

ER -

Arginine combination with fluoride and calcium glycerophosphate: effects of concentration and on biofilm fluid

Abstract

Funding

Keywords

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ASJC Scopus subject areas

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