Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells

Miina Ojansivu, Sari Vanhatupa, Leena Björkvik, Heikki Häkkänen, Minna Kellomäki, Reija Autio, Janne A. Ihalainen, Leena Hupa, Susanna Miettinen

    Research output: Contribution to journalArticleScientificpeer-review

    53 Citations (Scopus)

    Abstract

    Bioactive glasses are known for their ability to induce osteogenic differentiation of stem cells. To elucidate the mechanism of the osteoinductivity in more detail, we studied whether ionic extracts prepared from a commercial glass S53P4 and from three experimental glasses (2-06, 1-06 and 3-06) are alone sufficient to induce osteogenic differentiation of human adipose stem cells. Cells were cultured using basic medium or osteogenic medium as extract basis. Our results indicate that cells stay viable in all the glass extracts for the whole culturing period, 14 days. At 14 days the mineralization in osteogenic medium extracts was excessive compared to the control. Parallel to the increased mineralization we observed a decrease in the cell amount. Raman and Laser Induced Breakdown Spectroscopy analyses confirmed that the mineral consisted of calcium phosphates. Consistently, the osteogenic medium extracts also increased osteocalcin production and collagen Type-I accumulation in the extracellular matrix at 13 days. Of the four osteogenic medium extracts, 2-06 and 3-06 induced the best responses of osteogenesis. However, regardless of the enhanced mineral formation, alkaline phosphatase activity was not promoted by the extracts. The osteogenic medium extracts could potentially provide a fast and effective way to differentiate human adipose stem cells in vitro.

    Original languageEnglish
    Pages (from-to)190-203
    Number of pages14
    JournalActa Biomaterialia
    Volume21
    DOIs
    Publication statusPublished - 2015
    Publication typeA1 Journal article-refereed

    Keywords

    • Bioactive glass
    • Bone tissue engineering
    • Mesenchymal stem cell
    • Mineralization
    • Osteogenic differentiation

    Publication forum classification

    • Publication forum level 2

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