Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

Janne T. Koivisto, Tiina Joki, Jenny E. Parraga, Rami Paakkönen, Laura Yla-Outinen, Laura Salonen, Ilari Jönkkäri, Marja Peltola, Teemu O. Ihalainen, Susanna Narkilahti, Minna Kellomäki

    Research output: Contribution to journalArticleScientificpeer-review

    37 Citations (Scopus)

    Abstract

    Neural tissue engineering and three-dimensional in vitro tissue modeling require the development of biomaterials that take into account the specified requirements of human neural cells and tissue. In this study, an alternative method of producing biomimetic hydrogels based on gellan gum (GG) was developed by replacing traditional crosslinking methods with the bioamines spermidine and spermine. These bioamines were proven to function as crosslinkers for GG hydrogel at +37 °C, allowing for the encapsulation of human neurons. We studied the mechanical and rheological properties of the formed hydrogels, which showed biomimicking properties comparable to naïve rabbit brain tissue under physiologically relevant stress and strain. Human pluripotent stem cell-derived neuronal cells demonstrated good cytocompatibility in the GG-based hydrogels. Moreover, functionalization of GG hydrogels with laminin resulted in cell type-specific behavior: neuronal cell maturation and neurite migration.

    Original languageEnglish
    Article number025014
    JournalBiomedical Materials
    Volume12
    Issue number2
    DOIs
    Publication statusPublished - 2017
    Publication typeA1 Journal article-refereed

    Keywords

    • 3D cell culture
    • gellan gum
    • human pluripotent stem cells
    • hydrogel
    • laminin, neuronal cells

    Publication forum classification

    • Publication forum level 1

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