Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Basma Omar; Maie El-Gammal; Reda Abou-Shanab; Ioannis A. Fotidis; Irini Angelidaki; Yifeng Zhang

doi:10.1016/j.biortech.2019.121413

Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Basma Omar
, Maie El-Gammal
, Reda Abou-Shanab
, Ioannis A. Fotidis
, Irini Angelidaki
, Yifeng Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

54 Citations (Scopus)

Abstract

The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO₂ (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H₂ as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H₂/CO₂ gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H₂:1CO₂ ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.

Original language	English
Article number	121413
Journal	Bioresource Technology
Volume	286
DOIs	https://doi.org/10.1016/j.biortech.2019.121413
Publication status	Published - Aug 2019
Externally published	Yes
Publication type	A1 Journal article-refereed

Funding

Hector Garcia was thankful for technical support. Novo Nordisk Foundation (NNF16OC0021568) and Danish Council for Independent Research (DFF-1335-00142) had been corroborative financially this research. The Ministry of Higher Education of Egypt is gratefully acknowledged for PhD channel mission to the first author. The authors are grateful to Dr. Panagiotis Kougias and Dr. Laura Treu for helping in microbiological data analysis.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Acetogens
Acetyl-CoA pathway
Biogas upgrading
Gas fermentation
Microbial community

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Access to Document

10.1016/j.biortech.2019.121413

Cite this

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title = "Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition",

abstract = "The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H2/CO2 gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77\% (v/v))and acetate yield (72\%)were achieved for 2H2:1CO2 ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.",

keywords = "Acetogens, Acetyl-CoA pathway, Biogas upgrading, Gas fermentation, Microbial community",

author = "Basma Omar and Maie El-Gammal and Reda Abou-Shanab and Fotidis, \{Ioannis A.\} and Irini Angelidaki and Yifeng Zhang",

note = "Funding Information: Hector Garcia was thankful for technical support. Novo Nordisk Foundation (NNF16OC0021568) and Danish Council for Independent Research (DFF-1335-00142) had been corroborative financially this research. The Ministry of Higher Education of Egypt is gratefully acknowledged for PhD channel mission to the first author. The authors are grateful to Dr. Panagiotis Kougias and Dr. Laura Treu for helping in microbiological data analysis. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.biortech.2019.121413",

language = "English",

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journal = "Bioresource Technology",

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T2 - Impact of microbial community and gas composition

AU - Omar, Basma

AU - El-Gammal, Maie

AU - Abou-Shanab, Reda

AU - Fotidis, Ioannis A.

AU - Angelidaki, Irini

AU - Zhang, Yifeng

N1 - Funding Information: Hector Garcia was thankful for technical support. Novo Nordisk Foundation (NNF16OC0021568) and Danish Council for Independent Research (DFF-1335-00142) had been corroborative financially this research. The Ministry of Higher Education of Egypt is gratefully acknowledged for PhD channel mission to the first author. The authors are grateful to Dr. Panagiotis Kougias and Dr. Laura Treu for helping in microbiological data analysis. Publisher Copyright: © 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019/8

Y1 - 2019/8

N2 - The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H2/CO2 gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H2:1CO2 ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.

AB - The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H2/CO2 gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H2:1CO2 ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.

KW - Acetogens

KW - Acetyl-CoA pathway

KW - Biogas upgrading

KW - Gas fermentation

KW - Microbial community

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DO - 10.1016/j.biortech.2019.121413

M3 - Article

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SN - 0960-8524

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JO - Bioresource Technology

JF - Bioresource Technology

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ER -

Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

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