Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts

Susanna Nybond; Matti Karp; Teijo Yrjönen; Päivi Tammela

doi:10.1016/j.mimet.2015.04.014

Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts

Susanna Nybond
, Matti Karp
, Teijo Yrjönen
, Päivi Tammela^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

4 Citations (Scopus)

Abstract

We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.

Original language	English
Pages (from-to)	54-56
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	114
DOIs	https://doi.org/10.1016/j.mimet.2015.04.014
Publication status	Published - 1 Jul 2015
Publication type	A1 Journal article-refereed

Keywords

Antibacterial
Bioluminescence
Cell-based assay
Escherichia coli
Natural products
Screening

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)
Biotechnology

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10.1016/j.mimet.2015.04.014

Cite this

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abstract = "We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.",

keywords = "Antibacterial, Bioluminescence, Cell-based assay, Escherichia coli, Natural products, Screening",

author = "Susanna Nybond and Matti Karp and Teijo Yrj{\"o}nen and P{\"a}ivi Tammela",

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AU - Nybond, Susanna

AU - Karp, Matti

AU - Yrjönen, Teijo

AU - Tammela, Päivi

PY - 2015/7/1

Y1 - 2015/7/1

N2 - We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.

AB - We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.

KW - Antibacterial

KW - Bioluminescence

KW - Cell-based assay

KW - Escherichia coli

KW - Natural products

KW - Screening

UR - https://www.scopus.com/pages/publications/84928920094

U2 - 10.1016/j.mimet.2015.04.014

DO - 10.1016/j.mimet.2015.04.014

M3 - Article

AN - SCOPUS:84928920094

SN - 0167-7012

VL - 114

SP - 54

EP - 56

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts

Abstract

Keywords

Publication forum classification

ASJC Scopus subject areas

Access to Document

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