Cell Stretching Device for Live-Cell Confocal Microscopy

Joose Kreutzer, Marlitt Viehrig, Rolle Rahikainen, Antti Mäki, Vesa P. Hytönen, Pasi Kallio

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientific

    Abstract

    In this paper, we demonstrate a device with a stretchable cell cultivation substrate that is suitable for high-resolution confocal fluorescent microscopy imaging during mechanical stretching of cells. This small and innovative cell stretching device enables equiaxial strain (>10%) for cells. We demonstrate the capabilities of the device for semiautomatic real-time imaging of fluorescent fusion proteins localizing to actin fibers and nucleus of living cells. Furthermore, the stretching device is suitable for incubator conditions as it is fabricated solely from silicone and glass. Device fits to standard Petri dish frames that help the installation to a microscope for imaging. The stretching system provides different stretching waveforms, frequencies, and strain amplitudes.
    Original languageEnglish
    Title of host publication2016 IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC)
    Number of pages1
    ISBN (Electronic)978-1-4577-0220-4
    Publication statusPublished - Aug 2016
    Publication typeB3 Non-refereed article in conference proceedings
    EventAnnual International Conference of the IEEE Engineering in Medicine and Biology Society -
    Duration: 1 Jan 1900 → …

    Publication series

    NameAnnual International Conference of the Engineering in Medicine and Biology Society (EMBC)
    PublisherIEEE
    ISSN (Electronic)1558-4615

    Conference

    ConferenceAnnual International Conference of the IEEE Engineering in Medicine and Biology Society
    Period1/01/00 → …

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