In this paper, we demonstrate a device with a stretchable cell cultivation substrate that is suitable for high-resolution confocal fluorescent microscopy imaging during mechanical stretching of cells. This small and innovative cell stretching device enables equiaxial strain (>10%) for cells. We demonstrate the capabilities of the device for semiautomatic real-time imaging of fluorescent fusion proteins localizing to actin fibers and nucleus of living cells. Furthermore, the stretching device is suitable for incubator conditions as it is fabricated solely from silicone and glass. Device fits to standard Petri dish frames that help the installation to a microscope for imaging. The stretching system provides different stretching waveforms, frequencies, and strain amplitudes.
|Name||Annual International Conference of the Engineering in Medicine and Biology Society (EMBC)|
|Conference||Annual International Conference of the IEEE Engineering in Medicine and Biology Society|
|Period||1/01/00 → …|