Characterization of a novel bacterial cellulose producer for the production of eco-friendly piezoelectric-responsive films from a minimal medium containing waste carbon

Rahul Mangayil, Antti J. Rissanen, Arno Pammo, Dieval Guizelini, Pauli Losoi, Essi Sarlin, Sampo Tuukkanen, Ville Santala

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)
4 Downloads (Pure)

Abstract

Bacterial cellulose (BC) is a biodegradable polymer that benefits in purity, crystallinity and superior optical, structural and mechanical properties. Such properties facilitate BC to replace the conventional non-biodegradable materials used, for instance, in sensing applications. However, BC production is largely conducted in conventional medium containing model substrates and complex carbon-containing compounds. Aiming towards the production of eco-friendly piezoelectric-responsive BC films, we isolated and characterized a novel bacterial strain affiliated to Komagataeibacter rhaeticus. The K. rhaeticus ENS9a strain synthesized BC in minimal medium containing crude glycerol, generating a titer of 2.9 ± 0.3 g/L BC. This is, to the best of our knowledge, the highest BC titer reported from an unoptimized minimal medium containing crude glycerol. Interestingly, the films prepared from crude glycerol showed normal force and bending mode sensitivities of 6–11 pC/N and 40–71 pC/N, respectively, demonstrating a green platform to address both bioprocess waste valorization and implementation of cellulose-based alternatives for the non-sustainable and non-biodegradable materials, such as fluoropolymers or lead containing piezoceramics, used in sensing applications. In silico genome analysis predicted genes partaking in carbohydrate metabolism, BC biogenesis, and nitrogen fixation/regulation. Graphic abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)671–689
Number of pages19
JournalCellulose
Volume28
Issue number2
Early online date2020
DOIs
Publication statusPublished - 2021
Publication typeA1 Journal article-refereed

Keywords

  • Bacterial cellulose
  • Crude glycerol
  • Komagataeibacter spp
  • Piezoelectric material
  • Strain selection
  • Whole-genome analysis

Publication forum classification

  • Publication forum level 2

ASJC Scopus subject areas

  • Polymers and Plastics

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