Abstract
Komagataeibacter spp. has been used for the bioconversion of industrial wastes and lignocel-lulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in Komagataeibacter spp. In this study, we isolated a cellulose-producing bacterium capable of synthesiz-ing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to Komagataeibacter genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when K. rhaeticus ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.
Original language | English |
---|---|
Article number | 2230 |
Number of pages | 18 |
Journal | Microorganisms |
Volume | 9 |
Issue number | 11 |
DOIs | |
Publication status | Published - Oct 2021 |
Publication type | A1 Journal article-refereed |
Keywords
- Acetate
- Bacterial cellulose
- Crude glycerol
- Komagataeibacter rhaeticus
- Minimal medium
- Whole-genome analysis
Publication forum classification
- Publication forum level 1
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
- Virology
Fingerprint
Dive into the research topics of 'Characterization of komagataeibacter isolate reveals new prospects in waste stream valorization for bacterial cellulose production'. Together they form a unique fingerprint.Equipment
-
Synthetic biology
Santala, V. (Contact) & Santala, S. (Contact)
Materials Science and Environmental EngineeringFacility/equipment: Facility
-
Tampere Microscopy Center
Vippola, M. (Manager), Honkanen, M. (Operator) & Salminen, T. (Operator)
Faculty of Engineering and Natural SciencesFacility/equipment: Facility