TY - JOUR
T1 - Chemical modification strategies for viscosity-dependent processing of gellan gum
AU - Gering, Christine
AU - Rasheed, Anum
AU - Koivisto, Janne
AU - Parraga Meneses, Jenny
AU - Tuukkanen, Sampo
AU - Kellomäki, Minna
N1 - Funding Information:
This work was supported by the Academy of Finland through the Center of Excellence – Body on Chip ( 312409 , 326587 , 336663 ). C.G. received financial support from the Jenny and Antti Wihuri Foundation ( 3a3aec ) and A.R. from the TAU Doctoral School . We wish to thank Dr. Vijay Parihar for recording the 1 H NMR as well as the Wyatt Technology Corporation for successfully running the SEC and MALS, and Prof Michiel Postema for helpful discussion on rheology assessment.
Funding Information:
This work was supported by the Academy of Finland through the Center of Excellence ? Body on Chip (312409, 326587, 336663). C.G. received financial support from the Jenny and Antti Wihuri Foundation (3a3aec) and A.R. from the TAU Doctoral School. We wish to thank Dr. Vijay Parihar for recording the 1H NMR as well as the Wyatt Technology Corporation for successfully running the SEC and MALS, and Prof Michiel Postema for helpful discussion on rheology assessment.
PY - 2021/10
Y1 - 2021/10
N2 - Recently, the hydrogel-forming polysaccharide gellan gum (GG) has gained popularity as a versatile biomaterial for tissue engineering purposes. Here, we examine the modification strategies suitable for GG to overcome processing-related limitations. We emphasize the thorough assessment of the viscoelastic and mechanical properties of both precursor solutions and final hydrogels. The investigated modification strategies include purification, oxidation, reductive chain scission, and blending. We correlate polymer flow and hydrogel forming capabilities to viscosity-dependent methods including casting, injection and printing. Native GG and purified NaGG are shear thinning and feasible for printing, being similar in gelation and compression behavior. Oxidized GGox possesses reduced viscosity, higher toughness, and aldehydes as functional groups, while scissored GGsciss has markedly lower molecular weight. To exemplify extrudability, select modification products are printed using an extrusion-based bioprinter utilizing a crosslinker bath. Our robust modification strategies have widened the processing capabilities of GG without affecting its ability to form hydrogels.
AB - Recently, the hydrogel-forming polysaccharide gellan gum (GG) has gained popularity as a versatile biomaterial for tissue engineering purposes. Here, we examine the modification strategies suitable for GG to overcome processing-related limitations. We emphasize the thorough assessment of the viscoelastic and mechanical properties of both precursor solutions and final hydrogels. The investigated modification strategies include purification, oxidation, reductive chain scission, and blending. We correlate polymer flow and hydrogel forming capabilities to viscosity-dependent methods including casting, injection and printing. Native GG and purified NaGG are shear thinning and feasible for printing, being similar in gelation and compression behavior. Oxidized GGox possesses reduced viscosity, higher toughness, and aldehydes as functional groups, while scissored GGsciss has markedly lower molecular weight. To exemplify extrudability, select modification products are printed using an extrusion-based bioprinter utilizing a crosslinker bath. Our robust modification strategies have widened the processing capabilities of GG without affecting its ability to form hydrogels.
KW - Hydrogel
KW - Modified gellan gum
KW - Viscoelastic properties
KW - Mechanical testing
KW - Bioprinting
U2 - 10.1016/j.carbpol.2021.118335
DO - 10.1016/j.carbpol.2021.118335
M3 - Article
VL - 269
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
M1 - 118335
ER -