Chiral imaging of collagen by second-harmonic generation circular dichroism

H. Lee, M.J. Huttunen, K.-J. Hsu, M. Partanen, G.-Y. Zhuo, M. Kauranen, S.-W. Chu

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    Abstract

    We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and threedimensional orientation of collagen molecules.
    Translated title of the contributionChiral imaging of collagen by second-harmonic generation circular dichroism
    Original languageEnglish
    Pages (from-to)909-916
    Number of pages8
    JournalBiomedical Optics Express
    Volume4
    Issue number6
    DOIs
    Publication statusPublished - 2013
    Publication typeA1 Journal article-refereed

    Publication forum classification

    • Publication forum level 1

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