Chitosan/collagen/Mg, Se, Sr, Zn-substituted calcium phosphate scaffolds for bone tissue engineering applications: A growth factor free approach

Antonia Ressler, Roope Ohlsbom, Andreja Žužić, Arjen Gebraad, Erkka J. Frankberg, Toni Karri Pakarinen, Hrvoje Ivanković, Susanna Miettinen, Marica Ivanković

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)
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Abstract

According to the biomimetic bone scaffold design paradigm, a scaffold resembling natural bone tissue with molecular, structural and biological compatibility is needed to allow effective regeneration of bone tissue. Continuing our previous studies regarding scaffolds with chitosan matrix containing Mg, Se, Sr, Zn-substituted calcium phosphates (CaPs), the focus of this work was to further improve the properties of these growth factor-free scaffolds. By addition of collagen into the chitosan matrix at weight ratios of 100:0, 75:25, 50:50, 25:75 and 0:100, we aimed to better resemble natural bone tissue. Highly porous composite scaffolds based on chitosan and collagen, with 30 wt% of Mg, Se, Sr, Zn-substituted CaPs, were prepared by the freeze-gelation method. The scaffolds show a highly porous structure, with interconnected pores in the range of 20–350 μm and homogeneously dispersed CaPs. The added collagen further enhanced the stability measured during 28 days in simulated biological conditions. Live/dead and CyQUANT assays confirmed good viability and proliferation of human bone marrow-derived mesenchymal stem/stromal cells, while successful osteogenic differentiation was confirmed by alkaline phosphatase quantification and type I collagen immunocytochemical staining. Results indicated that the addition of collagen into the chitosan matrix containing Mg, Se, Sr, Zn-substituted CaPs improved the physicochemical and biological properties of the scaffolds.

Original languageEnglish
Article number112129
Number of pages14
JournalEuropean Polymer Journal
Volume194
DOIs
Publication statusPublished - 24 Jul 2023
Publication typeA1 Journal article-refereed

Keywords

  • Bone
  • Chitosan
  • Collagen
  • Ionic substitution
  • Lysozyme
  • Osteogenic differentiation
  • Regeneration
  • Scaffold

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • General Physics and Astronomy
  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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