Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Waldemar Kulig; Agnieszka Olzyńska; Piotr Jurkiewicz; Anu M. Kantola; Sanna Komulainen; Moutusi Manna; Mohsen Pourmousa; Mario Vazdar; Lukasz Cwiklik; Tomasz Rog; George Khelashvili; Daniel Harries; Ville Veikko Telkki; Martin Hof; Ilpo Vattulainen; Pavel Jungwirth

doi:10.1016/j.freeradbiomed.2015.03.006

Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Waldemar Kulig, Agnieszka Olzyńska, Piotr Jurkiewicz, Anu M. Kantola, Sanna Komulainen, Moutusi Manna, Mohsen Pourmousa, Mario Vazdar, Lukasz Cwiklik, Tomasz Rog, George Khelashvili, Daniel Harries, Ville Veikko Telkki, Martin Hof, Ilpo Vattulainen, Pavel Jungwirth

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

Abstract

The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized - tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.

Original language	English
Pages (from-to)	30-41
Number of pages	12
Journal	Free Radical Biology and Medicine
Volume	84
DOIs	https://doi.org/10.1016/j.freeradbiomed.2015.03.006
Publication status	Published - 1 Jul 2015
Publication type	A1 Journal article-refereed

Keywords

Phospholipid bilayers
Oxysterols
Molecular dynamics simulations
DPH anisotropy
NMR measurements
Laurdan fluorescence
Liposomes
Tilt modulus
FLUORESCENCE SOLVENT RELAXATION
MOLECULAR-DYNAMICS METHOD
MODEL MEMBRANES
FOURIER TRANSFORMATION
POTENTIAL FUNCTIONS
SOLVATION DYNAMICS
BENDING RIGIDITY
ORDER PARAMETERS
BILAYERS
PROTEINS

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Biochemistry
Physiology (medical)

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10.1016/j.freeradbiomed.2015.03.006

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Kulig, W., Olzyńska, A., Jurkiewicz, P., Kantola, A. M., Komulainen, S., Manna, M., Pourmousa, M., Vazdar, M., Cwiklik, L., Rog, T., Khelashvili, G., Harries, D., Telkki, V. V., Hof, M., Vattulainen, I., & Jungwirth, P. (2015). Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols. Free Radical Biology and Medicine, 84, 30-41. https://doi.org/10.1016/j.freeradbiomed.2015.03.006

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title = "Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols",

abstract = "The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized - tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.",

keywords = "Phospholipid bilayers, Oxysterols, Molecular dynamics simulations, DPH anisotropy, NMR measurements, Laurdan fluorescence, Liposomes, Tilt modulus, FLUORESCENCE SOLVENT RELAXATION, MOLECULAR-DYNAMICS METHOD, MODEL MEMBRANES, FOURIER TRANSFORMATION, POTENTIAL FUNCTIONS, SOLVATION DYNAMICS, BENDING RIGIDITY, ORDER PARAMETERS, BILAYERS, PROTEINS",

author = "Waldemar Kulig and Agnieszka Olzy{\'n}ska and Piotr Jurkiewicz and Kantola, {Anu M.} and Sanna Komulainen and Moutusi Manna and Mohsen Pourmousa and Mario Vazdar and Lukasz Cwiklik and Tomasz Rog and George Khelashvili and Daniel Harries and Telkki, {Ville Veikko} and Martin Hof and Ilpo Vattulainen and Pavel Jungwirth",

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doi = "10.1016/j.freeradbiomed.2015.03.006",

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Kulig, W, Olzyńska, A, Jurkiewicz, P, Kantola, AM, Komulainen, S, Manna, M, Pourmousa, M, Vazdar, M, Cwiklik, L, Rog, T, Khelashvili, G, Harries, D, Telkki, VV, Hof, M, Vattulainen, I & Jungwirth, P 2015, 'Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols', Free Radical Biology and Medicine, vol. 84, pp. 30-41. https://doi.org/10.1016/j.freeradbiomed.2015.03.006

TY - JOUR

T1 - Cholesterol under oxidative stress

T2 - How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

AU - Kulig, Waldemar

AU - Olzyńska, Agnieszka

AU - Jurkiewicz, Piotr

AU - Kantola, Anu M.

AU - Komulainen, Sanna

AU - Manna, Moutusi

AU - Pourmousa, Mohsen

AU - Vazdar, Mario

AU - Cwiklik, Lukasz

AU - Rog, Tomasz

AU - Khelashvili, George

AU - Harries, Daniel

AU - Telkki, Ville Veikko

AU - Hof, Martin

AU - Vattulainen, Ilpo

AU - Jungwirth, Pavel

PY - 2015/7/1

Y1 - 2015/7/1

N2 - The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized - tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.

AB - The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized - tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.

KW - Phospholipid bilayers

KW - Oxysterols

KW - Molecular dynamics simulations

KW - DPH anisotropy

KW - NMR measurements

KW - Laurdan fluorescence

KW - Liposomes

KW - Tilt modulus

KW - FLUORESCENCE SOLVENT RELAXATION

KW - MOLECULAR-DYNAMICS METHOD

KW - MODEL MEMBRANES

KW - FOURIER TRANSFORMATION

KW - POTENTIAL FUNCTIONS

KW - SOLVATION DYNAMICS

KW - BENDING RIGIDITY

KW - ORDER PARAMETERS

KW - BILAYERS

KW - PROTEINS

UR - http://www.scopus.com/inward/record.url?scp=84928530055&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2015.03.006

DO - 10.1016/j.freeradbiomed.2015.03.006

M3 - Article

SN - 0891-5849

VL - 84

SP - 30

EP - 41

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

ER -

Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Abstract

Keywords

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ASJC Scopus subject areas

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