Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks

Fikret Emre Kapucu; Andrey Vinogradov; Tanja Hyvärinen; Laura Ylä-Outinen; Susanna Narkilahti

doi:10.1038/s41597-022-01242-4

Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks

Fikret Emre Kapucu^*
, Andrey Vinogradov
, Tanja Hyvärinen
, Laura Ylä-Outinen
, Susanna Narkilahti

^*Corresponding author for this work

Research output: Contribution to journal › Data article › peer-review

18 Citations (Scopus)

54 Downloads (Pure)

Abstract

We present a dataset of microelectrode array (MEA) recordings from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during their in vitro maturation. The data were prepared to assess extracellularly recorded spontaneous activity and to compare the functional development of these neuronal networks. In addition to recordings of spontaneous activity, we provide pharmacological responses of hPSC-derived and rat cortical cultures at their mature stage. Together with the recorded electrode raw data, we share the analysis code to form a comprehensive dataset including spike times, spike waveforms, burst activity and network synchronization metrics calculated with two different connectivity estimators. Moreover, we provide the analysis code that produced the key scientific findings published previously with this dataset. This large dataset enables investigation of the functional aspects of maturing cortical neuronal networks and provides substantial parameters to assess the differences and similarities between hPSC-derived and rat cortical networks in vitro. This publicly available dataset will be beneficial, especially for experimental and computational neuroscientists.

Original language	English
Article number	120
Number of pages	10
Journal	Scientific Data
Volume	9
DOIs	https://doi.org/10.1038/s41597-022-01242-4
Publication status	Published - Mar 2022
Publication type	A1 Journal article-refereed

Funding

This work was supported by the Academy of Finland (grant number 332693 (FEK), 286990/326436 (LYO), 312414 and 311017 (SN)) and Orion Research Foundation (grant for postdoctoral research 2019 (FEK), grant for doctoral dissertation work 2020 (AV)). The original MEA data was produced with the help of Tampere facility of Electrophysiological Measurements.

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

Access to Document

10.1038/s41597-022-01242-4Licence: CC BY

s41597-022-01242-4Final published version, 2.66 MBLicence: CC BY

https://urn.fi/URN:NBN:fi:tuni-202205034253Licence: CC BY

Comparative microelectrode array dataset of the functional development of hPSC-derived and rat neuronal networks
Kapucu, F. E. (Creator), Vinogradov, A. (Creator), Hyvärinen, T. (Creator), Ylä-Outinen, L. (Creator) & Narkilahti, S. (Creator), G-Node, 7 Mar 2021
DOI: 10.12751/g-node.wvr3jf
Dataset

Electrophysiological Measurements
Narkilahti, S. (Manager)
Facility/equipment: Research infrastructure

Cite this

@article{b5409558e5bf422cbc17b451cdae088c,

title = "Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks",

abstract = "We present a dataset of microelectrode array (MEA) recordings from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during their in vitro maturation. The data were prepared to assess extracellularly recorded spontaneous activity and to compare the functional development of these neuronal networks. In addition to recordings of spontaneous activity, we provide pharmacological responses of hPSC-derived and rat cortical cultures at their mature stage. Together with the recorded electrode raw data, we share the analysis code to form a comprehensive dataset including spike times, spike waveforms, burst activity and network synchronization metrics calculated with two different connectivity estimators. Moreover, we provide the analysis code that produced the key scientific findings published previously with this dataset. This large dataset enables investigation of the functional aspects of maturing cortical neuronal networks and provides substantial parameters to assess the differences and similarities between hPSC-derived and rat cortical networks in vitro. This publicly available dataset will be beneficial, especially for experimental and computational neuroscientists.",

author = "Kapucu, \{Fikret Emre\} and Andrey Vinogradov and Tanja Hyv{\"a}rinen and Laura Yl{\"a}-Outinen and Susanna Narkilahti",

note = "Funding Information: This work was supported by the Academy of Finland (grant number 332693 (FEK), 286990/326436 (LYO), 312414 and 311017 (SN)) and Orion Research Foundation (grant for postdoctoral research 2019 (FEK), grant for doctoral dissertation work 2020 (AV)). The original MEA data was produced with the help of Tampere facility of Electrophysiological Measurements. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

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doi = "10.1038/s41597-022-01242-4",

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T1 - Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks

AU - Kapucu, Fikret Emre

AU - Vinogradov, Andrey

AU - Hyvärinen, Tanja

AU - Ylä-Outinen, Laura

AU - Narkilahti, Susanna

N1 - Funding Information: This work was supported by the Academy of Finland (grant number 332693 (FEK), 286990/326436 (LYO), 312414 and 311017 (SN)) and Orion Research Foundation (grant for postdoctoral research 2019 (FEK), grant for doctoral dissertation work 2020 (AV)). The original MEA data was produced with the help of Tampere facility of Electrophysiological Measurements. Publisher Copyright: © 2022, The Author(s).

PY - 2022/3

Y1 - 2022/3

N2 - We present a dataset of microelectrode array (MEA) recordings from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during their in vitro maturation. The data were prepared to assess extracellularly recorded spontaneous activity and to compare the functional development of these neuronal networks. In addition to recordings of spontaneous activity, we provide pharmacological responses of hPSC-derived and rat cortical cultures at their mature stage. Together with the recorded electrode raw data, we share the analysis code to form a comprehensive dataset including spike times, spike waveforms, burst activity and network synchronization metrics calculated with two different connectivity estimators. Moreover, we provide the analysis code that produced the key scientific findings published previously with this dataset. This large dataset enables investigation of the functional aspects of maturing cortical neuronal networks and provides substantial parameters to assess the differences and similarities between hPSC-derived and rat cortical networks in vitro. This publicly available dataset will be beneficial, especially for experimental and computational neuroscientists.

AB - We present a dataset of microelectrode array (MEA) recordings from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during their in vitro maturation. The data were prepared to assess extracellularly recorded spontaneous activity and to compare the functional development of these neuronal networks. In addition to recordings of spontaneous activity, we provide pharmacological responses of hPSC-derived and rat cortical cultures at their mature stage. Together with the recorded electrode raw data, we share the analysis code to form a comprehensive dataset including spike times, spike waveforms, burst activity and network synchronization metrics calculated with two different connectivity estimators. Moreover, we provide the analysis code that produced the key scientific findings published previously with this dataset. This large dataset enables investigation of the functional aspects of maturing cortical neuronal networks and provides substantial parameters to assess the differences and similarities between hPSC-derived and rat cortical networks in vitro. This publicly available dataset will be beneficial, especially for experimental and computational neuroscientists.

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DO - 10.1038/s41597-022-01242-4

M3 - Data article

C2 - 35354837

AN - SCOPUS:85127275551

SN - 2052-4463

VL - 9

JO - Scientific Data

JF - Scientific Data

M1 - 120

ER -

Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks

Abstract

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Datasets

Comparative microelectrode array dataset of the functional development of hPSC-derived and rat neuronal networks

Equipment

Electrophysiological Measurements

Cite this