Comparative proteomics analysis of the mouse mini-gut organoid: insights into markers of gluten challenge from celiac disease intestinal biopsies

Robert Moulder, Santosh D. Bhosale, Keijo Viiri, Riitta Lahesmaa

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Introduction: Organoid models enable three-dimensional representation of cellular systems, providing flexible and accessible research tools, and can highlight key biomolecules. Such models of the intestinal epithelium can provide significant knowledge for the study of celiac disease and provide an additional context for the nature of markers observed from patient biopsy data. Methods: Using LC–MS/MS, the proteomes of the crypt and enterocyte-like states of a mouse mini-gut organoid model were measured. The data were further compared with published biopsy data by comparing the changes induced by gluten challenge after a gluten-free diet. Results and discussion: These analyses identified 4,850 protein groups and revealed how 400 putative biomarkers of dietary challenge were differentially expressed in the organoid model. In addition to the extensive changes within the differentiated cells, the data reiterated the disruption of the crypt–villus axis after gluten challenge. The mass spectrometry data are available via ProteomeXchange with the identifier PXD025690.

Original languageEnglish
Article number1446822
Number of pages15
JournalFrontiers in Molecular Biosciences
Volume11
DOIs
Publication statusPublished - 2024
Publication typeA1 Journal article-refereed

Keywords

  • celiac disease
  • gluten challenge
  • mini-gut
  • organoid
  • proteomics

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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