Computational study of structural changes in neuronal networks during growth: a model of dissociated neocortical cultures

Jugoslava Acimovic, Tuomo Mäki-Marttunen, Marja-Leena Linne

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    Abstract

    Networks of neurons possess distinct structural organization that constraints generated activity patterns, and consequently, the functions of the system. The emergence of the network structure can be understood by studying the rules that govern growth of neurons and their self-organization into neuronal circuits. We analyze these rules using a computational model of growth developed for dissociated neocortical cultures. Compared to the growth in vivo, the cultures represent simplified two dimensional systems that still possess the intrinsic properties of single neurons although they lack the natural extracellular environment present in vivo. This setup provides a possibility to address in depth the selected mechanisms that affect neuronal growth. The collected structural data (through staining and microscopy) and electrophysiological data (using microelectrode arrays) facilitate validation of computational models. Neuronal growth in dissociated cultures has been examined in several studies in order to access the role of activity in network development [6],[7] or to extract the structural changes during growth from the recorded activity and identify the significant time points in network development [4]. In addition, two simulators of neuronal growth were recently published to aid the development of computational models [3],[9]. Their performance, in context of modeling neocortical cultures, is compared in [1].

    The analyzed model consists of two types on neurons, most commonly observed in the neocortical cultures, the pyramidal cells and the nonpyramidal GABAergic cells, placed in a dish-like space with the density of cells corresponding to the experimental values. The phenomenological model that takes into account growth of every neurite is constructed using the description from the literature [3],[8]. It is compared to the model that defines only the overall shape of each neuritic field. We examine the critical time point in network development, i.e. the emergence of fully connected networks [2],[4], which is dependent on the overall growth speed of neurites. The local structural features are accessed using the frequency of motifs in networks [2],[5]. Local connectivity patterns, captured by the motif counts, depend on the shape of neurites and distribution of synaptic contacts along neurites. The goal of this study is to analyze model dynamics through evaluation of the proposed measures. The dependence on model parameters is examined in details, particularly, whether small variations in parameter values significantly affect both measures of network structure. The obtained conclusions are compared to the experimental findings from the literature [4, 5].
    Translated title of the contributionComputational study of structural changes in neuronal networks during growth: a model of dissociated neocortical cultures
    Original languageEnglish
    Title of host publicationTwentieth Annual Computational Neuroscience Meeting: CNS*2011
    EditorsJean-Marc Fellous, Astrid Prinz
    Place of PublicationStockholm
    PublisherBioMed Central
    Chaptervolume 12 (Suppl 1)
    PagesP203
    Number of pages1
    Volume12 (Suppl 1)
    DOIs
    Publication statusPublished - 2011
    Publication typeA4 Article in a conference publication
    EventTwentieth Annual Computational Neuroscience Meeting: CNS*2011 - Stockholm, Sweden
    Duration: 23 Jul 201128 Jul 2011

    Publication series

    NameAnnual Computational Neuroscience Meeting CNS
    PublisherBioMed Central
    Volume12
    ISSN (Print)1471-2202

    Conference

    ConferenceTwentieth Annual Computational Neuroscience Meeting: CNS*2011
    CountrySweden
    CityStockholm
    Period23/07/1128/07/11

    Keywords

    • computational model
    • pyramidal cell
    • synaptic contact
    • network development
    • neuronal circuit

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • Neuroscience (miscellaneous)

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