COX-2 regulates the proliferation of glioma stem like cells

Vivek Sharma, Deobrat Dixit, Sadashib Ghosh, Ellora Sen

    Research output: Contribution to journalArticleScientificpeer-review

    41 Citations (Scopus)


    Cancer stem-like cells (CSCs) possessing features of neural precursor cells (NPC) influence initiation, recurrence and chemoresistance of glioblastoma multiforme (GBM). As inflammation is crucial for glioblastoma progression we investigated the effect of chronic IL-1β treatment on CSCs derived from glioblastoma cell line U87MG. Exposure to IL-1β for 10 days increased (i) accumulation of 8-OHdG - a key biomarker of oxidative DNA damage; (ii) DNA damage response (DDR) indicators γH2AX, ATM and DNA-PK; (iii) nuclear and cytoplasmic p53 and COX-2 levels and (iv) interaction between COX-2 and p53. Despite upregulating p53 expression IL-1β had no effect on cell cycle progression, apoptosis or self renewal capacity of CSCs. COX-2 inhibitor Celecoxib reduced self renewal capacity and increased apoptosis of both control and IL-1β treated CSCs. Therefore the ability of COX-2 to regulate proliferation of CSCs irrespective of exposure to IL-1β, warrants further investigation of COX-2 as a potential anti-glioma target.

    Original languageEnglish
    Pages (from-to)567-571
    Number of pages5
    Issue number5
    Publication statusPublished - Oct 2011
    Publication typeA1 Journal article-refereed


    • COX-2
    • Glioblastoma
    • IL-1β
    • p53

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience
    • Cell Biology


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