Cysteine-rich protein 1 is regulated by transforming growth factor-β1 and expressed in lung fibrosis

Päivi M. Järvinen, Marjukka Myllärniemi, Hester Liu, Henna M. Moore, Outi Leppäranta, Kaisa Salmenkivi, Katri Koli, Leena Latonen, Arja M. Band, Marikki Laiho

    Research output: Contribution to journalArticleScientificpeer-review

    7 Citations (Scopus)

    Abstract

    Transforming growth factor-β (TGF-β) is a diverse cytokine regulating growth, apoptosis, differentiation, adhesion, invasion, and extracellular matrix production. Dysregulation of TGF-β is associated with fibrotic disorders and epithelial-mesenchymal transition, and has been linked with idiopathic pulmonary fibrosis (IPF). Cysteine-rich protein 1 (CRP1) is a small LIM-domain containing protein involved in smooth muscle differentiation. Here, we show that TGF-β1 increases the expression of CRP1 protein and that CRP1 levels increase in a biphasic fashion. A rapid transient (15-45min) increase in CRP1 is followed by a subsequent, sustained increase in CRP1 a few hours afterwards that lasts several days. We find that TGF-β1 regulates the expression of CRP1 through Smad and non-conventional p38 MAPK signaling pathways in a transcription-independent manner and that the induction occurs concomitant with an increase in myofibroblast differentiation. Using CRP1 silencing by shRNA, we identify CRP1 as a novel factor mediating cell contractility. Furthermore, we localize CRP1 to fibroblastic foci in IPF lungs and find that CRP1 is significantly more expressed in IPF as compared to control lung tissue. The results show that CRP1 is a novel TGF-β1 regulated protein that is expressed in fibrotic lesions and may be relevant in the IPF disease.

    Original languageEnglish
    Pages (from-to)2605-2612
    Number of pages8
    JournalJournal of Cellular Physiology
    Volume227
    Issue number6
    DOIs
    Publication statusPublished - 2012
    Publication typeA1 Journal article-refereed

    Publication forum classification

    • Publication forum level 2

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