Discrimination and cell counting of profile of superoxide dismutase (SOD) under hypercholesterolemia using K-means clustering

Clove leaf extract contains high natural antioxidant.In this study, an experiment was conducted to analyse theeffect methanol extract of clove leaf on the profile of superoxidedismutase (SOD) in the rabbits liver under hypercholesterolemiccondition. Rabbits were divided into three groups i.e (1) negativecontrol group, (2) positive control (hypercholesterolemic) group,which fed diet containing 1% cholesterol for 50 days, and (3)group which was given clove leaf extract and 1% cholesterolsimultaneously for 50 days. Contents profile of antioxidantsuperoxide dismutase in rabbit liver tissue was obtained usingimmunihistochemical techniques. The images were taken fromeach group using a microscope to analyse the reaction of cloveleaf extract to the cells. The images were processed using K-mean clustering to discriminate the cells after treatment. The cellsegmentation was performed to count number of the cells basedon their treatment. The cell counting technique then comparedwith the manual counting. The results demonstrate that thedeveloped technique was a reliable image processing techniquewhich could be used in the cell counting purposes.