DNA-origami-directed virus capsid polymorphism

Iris Seitz; Sharon Saarinen; Esa-Pekka Kumpula; Donna McNeale; Eduardo Anaya-Plaza; Vili Lampinen; Vesa P. Hytönen; Frank Sainsbury; Jeroen J.L.M. Cornelissen; Veikko Linko; Juha T. Huiskonen; Mauri A. Kostiainen

doi:10.1038/s41565-023-01443-x

DNA-origami-directed virus capsid polymorphism

Iris Seitz
, Sharon Saarinen
, Esa-Pekka Kumpula
, Donna McNeale
, Eduardo Anaya-Plaza
, Vili Lampinen
, Vesa P. Hytönen
, Frank Sainsbury
, Jeroen J.L.M. Cornelissen
, Veikko Linko
, Juha T. Huiskonen^*
, Mauri A. Kostiainen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

36 Citations (Scopus)

32 Downloads (Pure)

Abstract

Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA–DNA origami structures to pave way for next-generation cargo protection and targeting strategies.

Original language	English
Pages (from-to)	1205-1212
Number of pages	11
Journal	Nature Nanotechnology
Volume	18
Early online date	Jul 2023
DOIs	https://doi.org/10.1038/s41565-023-01443-x
Publication status	Published - 2023
Publication type	A1 Journal article-refereed

Publication forum classification

Publication forum level 3

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
Biomedical Engineering
General Materials Science
Condensed Matter Physics
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41565-023-01443-xLicence: CC BY

s41565-023-01443-xFinal published version, 4.66 MBLicence: CC BY

https://urn.fi/URN:NBN:fi:tuni-202309067988Licence: CC BY

Helical shell of CCMV capsid protein on DNA origami 6HB-2k
Seitz, I. (Contributor), Saarinen, S. (Contributor), Kumpula, E.-P. (Contributor), McNeale, D. (Contributor), Anaya-Plaza, E. (Contributor), Lampinen, V. (Creator), Hytönen, V. P. (Contributor), Sainsbury, F. (Contributor), Cornelissen, J. J. L. M. (Contributor), Linko, V. (Contributor), Huiskonen, J. T. (Contributor) & Kostiainen, M. A. (Contributor), Protein Data Bank (PDB), 5 Jul 2023
DOI: 10.2210/pdb8bi4/pdb, https://www.ebi.ac.uk/pdbe/entry/pdb/8bi4
Dataset

Cite this

@article{44e58ba8dca34c6e985ed34bab02bfad,

title = "DNA-origami-directed virus capsid polymorphism",

abstract = "Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA–DNA origami structures to pave way for next-generation cargo protection and targeting strategies.",

author = "Iris Seitz and Sharon Saarinen and Esa-Pekka Kumpula and Donna McNeale and Eduardo Anaya-Plaza and Vili Lampinen and Hyt{\"o}nen, \{Vesa P.\} and Frank Sainsbury and Cornelissen, \{Jeroen J.L.M.\} and Veikko Linko and Huiskonen, \{Juha T.\} and Kostiainen, \{Mauri A.\}",

note = "Funding Information: The authors acknowledge financial support from the European Research Council (ERC) and ERA Chair MATTER under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement numbers 101002258 (M.A.K.) and 856705 (V. Linko)), the Emil Aaltonen Foundation (V. Linko), the Sigrid Jus{\'e}lius Foundation (V. Linko), the Academy of Finland (grant numbers 341057 (E.A.-P.) and 314671 (M.A.K)), the Finnish Foundation for Technology Promotion (V. Lampinen), and the Jane and Aatos Erkko Foundation (V. Linko and M.A.K.). This work was carried out under the Academy of Finland Centers of Excellence Program (2022-2029) in Life-Inspired Hybrid Materials (LIBER), project number number 346110 (M.A.K.). K. M. Nguyen and A. Kuzyk are acknowledged for providing the staple strands for the 13HR sample, P. Laurinm{\"a}ki and B. L{\"o}flund for technical assistance with cryo-EM, M. Hankaniemi for support with NoV production, and M. Sammalkorpi and A. Scacchi for technical discussions. The facilities and expertise of the HiLIFE cryo-EM unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct and Biocenter Finland are gratefully acknowledged. The authors also acknowledge CSC–IT Center of Science, Finland, for computational resources, Biocenter Finland for support of protein production and characterization infrastructure, and the provision of facilities and technical support by Aalto University Bioeconomy Facilities, OtaNanoNanomicroscopy Center (Aalto-NMC) and Micronova Nanofabrication Center. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

doi = "10.1038/s41565-023-01443-x",

language = "English",

volume = "18",

pages = "1205--1212",

journal = "Nature Nanotechnology",

issn = "1748-3387",

publisher = "Nature Research",

}

TY - JOUR

T1 - DNA-origami-directed virus capsid polymorphism

AU - Seitz, Iris

AU - Saarinen, Sharon

AU - Kumpula, Esa-Pekka

AU - McNeale, Donna

AU - Anaya-Plaza, Eduardo

AU - Lampinen, Vili

AU - Hytönen, Vesa P.

AU - Sainsbury, Frank

AU - Cornelissen, Jeroen J.L.M.

AU - Linko, Veikko

AU - Huiskonen, Juha T.

AU - Kostiainen, Mauri A.

N1 - Funding Information: The authors acknowledge financial support from the European Research Council (ERC) and ERA Chair MATTER under the European Union’s Horizon 2020 research and innovation programme (grant agreement numbers 101002258 (M.A.K.) and 856705 (V. Linko)), the Emil Aaltonen Foundation (V. Linko), the Sigrid Jusélius Foundation (V. Linko), the Academy of Finland (grant numbers 341057 (E.A.-P.) and 314671 (M.A.K)), the Finnish Foundation for Technology Promotion (V. Lampinen), and the Jane and Aatos Erkko Foundation (V. Linko and M.A.K.). This work was carried out under the Academy of Finland Centers of Excellence Program (2022-2029) in Life-Inspired Hybrid Materials (LIBER), project number number 346110 (M.A.K.). K. M. Nguyen and A. Kuzyk are acknowledged for providing the staple strands for the 13HR sample, P. Laurinmäki and B. Löflund for technical assistance with cryo-EM, M. Hankaniemi for support with NoV production, and M. Sammalkorpi and A. Scacchi for technical discussions. The facilities and expertise of the HiLIFE cryo-EM unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct and Biocenter Finland are gratefully acknowledged. The authors also acknowledge CSC–IT Center of Science, Finland, for computational resources, Biocenter Finland for support of protein production and characterization infrastructure, and the provision of facilities and technical support by Aalto University Bioeconomy Facilities, OtaNanoNanomicroscopy Center (Aalto-NMC) and Micronova Nanofabrication Center. Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA–DNA origami structures to pave way for next-generation cargo protection and targeting strategies.

AB - Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA–DNA origami structures to pave way for next-generation cargo protection and targeting strategies.

U2 - 10.1038/s41565-023-01443-x

DO - 10.1038/s41565-023-01443-x

M3 - Article

AN - SCOPUS:85164964063

SN - 1748-3387

VL - 18

SP - 1205

EP - 1212

JO - Nature Nanotechnology

JF - Nature Nanotechnology

ER -

DNA-origami-directed virus capsid polymorphism

Abstract

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ASJC Scopus subject areas

UN SDGs

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Helical shell of CCMV capsid protein on DNA origami 6HB-2k

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