Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells

Clarisse Ribeiro, Jenita Pärssinen, Vítor Sencadas, Vítor Correia, S. Miettinen, Vesa P. Hytönen, Senentxu Lanceros-Méndez

Research output: Contribution to journalArticleScientificpeer-review

88 Citations (Scopus)

Abstract

This work reports on the influence of the substrate polarization of electroactive β-poly(vinylidene fluoride) (β-PVDF) on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and "poled -") adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. "Poled -" β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.

Original languageEnglish
Pages (from-to)2172-2175
Number of pages4
JournalJournal of Biomedical Materials Research. Part A
Volume103
Issue number6
DOIs
Publication statusPublished - 2015
Publication typeA1 Journal article-refereed

Keywords

  • Cell adhesion
  • dynamic cell culture
  • dynamic conditions
  • electroactive polymer
  • osteogenesis
  • stem cell

Publication forum classification

  • Publication forum level 2

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