Effect of different ammonia sources on aceticlastic and hydrogenotrophic methanogens

Hailin Tian; Ioannis A. Fotidis; Konstantinos Kissas; Irini Angelidaki

doi:10.1016/j.biortech.2017.11.081

Effect of different ammonia sources on aceticlastic and hydrogenotrophic methanogens

Hailin Tian
, Ioannis A. Fotidis^*
, Konstantinos Kissas
, Irini Angelidaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

92 Citations (Scopus)

Abstract

Ammonium chloride (NH₄Cl) was usually used as a model ammonia source to simulate ammonia inhibition during anaerobic digestion (AD) of nitrogen-rich feedstocks. However, ammonia in AD originates mainly from degradation of proteins, urea and nucleic acids, which is distinct from NH₄Cl. Thus, in this study, the inhibitory effect of a “natural” ammonia source (urea) and NH₄Cl, on four pure methanogenic strains (aceticlastic: Methanosarcina thermophila, Methanosarcina barkeri; hydrogenotrophic: Methanoculleus bourgensis, Methanoculleus thermophilus), was assessed under mesophilic (37 °C) and thermophilic (55 °C) conditions. The results showed that urea hydrolysis increased pH significantly to unsuitable levels for methanogenic growth, while NH₄Cl had a negligible effect on pH. After adjusting initial pH to 7 and 8, urea was significantly stronger inhibitor with longer lag phases to methanogenesis compared to NH₄Cl. Overall, urea seems to be more toxic on both aceticlastic and hydrogenotrophic methanogens compared to NH₄Cl under the same total and free ammonia levels.

Original language	English
Pages (from-to)	390-397
Number of pages	8
Journal	Bioresource Technology
Volume	250
DOIs	https://doi.org/10.1016/j.biortech.2017.11.081
Publication status	Published - Feb 2018
Externally published	Yes
Publication type	A1 Journal article-refereed

Funding

This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH 3 -Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327). Hailin Tian would like to thank for the financial support from China Scholarship Council , and Konstantinos Kissas thanks Alexander S. Onassis Public Benefit Foundation for granting a scholarship. The authors thank Hector Garcia for his technical support during the experiments.

Keywords

Ammonia inhibition
Ammonium chloride
Anaerobic digestion
Pure strain
Urea

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2017.11.081

Cite this

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title = "Effect of different ammonia sources on aceticlastic and hydrogenotrophic methanogens",

abstract = "Ammonium chloride (NH4Cl) was usually used as a model ammonia source to simulate ammonia inhibition during anaerobic digestion (AD) of nitrogen-rich feedstocks. However, ammonia in AD originates mainly from degradation of proteins, urea and nucleic acids, which is distinct from NH4Cl. Thus, in this study, the inhibitory effect of a “natural” ammonia source (urea) and NH4Cl, on four pure methanogenic strains (aceticlastic: Methanosarcina thermophila, Methanosarcina barkeri; hydrogenotrophic: Methanoculleus bourgensis, Methanoculleus thermophilus), was assessed under mesophilic (37 °C) and thermophilic (55 °C) conditions. The results showed that urea hydrolysis increased pH significantly to unsuitable levels for methanogenic growth, while NH4Cl had a negligible effect on pH. After adjusting initial pH to 7 and 8, urea was significantly stronger inhibitor with longer lag phases to methanogenesis compared to NH4Cl. Overall, urea seems to be more toxic on both aceticlastic and hydrogenotrophic methanogens compared to NH4Cl under the same total and free ammonia levels.",

keywords = "Ammonia inhibition, Ammonium chloride, Anaerobic digestion, Pure strain, Urea",

author = "Hailin Tian and Fotidis, \{Ioannis A.\} and Konstantinos Kissas and Irini Angelidaki",

note = "Funding Information: This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH 3 -Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327). Hailin Tian would like to thank for the financial support from China Scholarship Council , and Konstantinos Kissas thanks Alexander S. Onassis Public Benefit Foundation for granting a scholarship. The authors thank Hector Garcia for his technical support during the experiments. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "2018",

month = feb,

doi = "10.1016/j.biortech.2017.11.081",

language = "English",

volume = "250",

pages = "390--397",

journal = "Bioresource Technology",

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AU - Tian, Hailin

AU - Fotidis, Ioannis A.

AU - Kissas, Konstantinos

AU - Angelidaki, Irini

N1 - Funding Information: This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH 3 -Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327). Hailin Tian would like to thank for the financial support from China Scholarship Council , and Konstantinos Kissas thanks Alexander S. Onassis Public Benefit Foundation for granting a scholarship. The authors thank Hector Garcia for his technical support during the experiments. Publisher Copyright: © 2017 Elsevier Ltd Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2018/2

Y1 - 2018/2

N2 - Ammonium chloride (NH4Cl) was usually used as a model ammonia source to simulate ammonia inhibition during anaerobic digestion (AD) of nitrogen-rich feedstocks. However, ammonia in AD originates mainly from degradation of proteins, urea and nucleic acids, which is distinct from NH4Cl. Thus, in this study, the inhibitory effect of a “natural” ammonia source (urea) and NH4Cl, on four pure methanogenic strains (aceticlastic: Methanosarcina thermophila, Methanosarcina barkeri; hydrogenotrophic: Methanoculleus bourgensis, Methanoculleus thermophilus), was assessed under mesophilic (37 °C) and thermophilic (55 °C) conditions. The results showed that urea hydrolysis increased pH significantly to unsuitable levels for methanogenic growth, while NH4Cl had a negligible effect on pH. After adjusting initial pH to 7 and 8, urea was significantly stronger inhibitor with longer lag phases to methanogenesis compared to NH4Cl. Overall, urea seems to be more toxic on both aceticlastic and hydrogenotrophic methanogens compared to NH4Cl under the same total and free ammonia levels.

AB - Ammonium chloride (NH4Cl) was usually used as a model ammonia source to simulate ammonia inhibition during anaerobic digestion (AD) of nitrogen-rich feedstocks. However, ammonia in AD originates mainly from degradation of proteins, urea and nucleic acids, which is distinct from NH4Cl. Thus, in this study, the inhibitory effect of a “natural” ammonia source (urea) and NH4Cl, on four pure methanogenic strains (aceticlastic: Methanosarcina thermophila, Methanosarcina barkeri; hydrogenotrophic: Methanoculleus bourgensis, Methanoculleus thermophilus), was assessed under mesophilic (37 °C) and thermophilic (55 °C) conditions. The results showed that urea hydrolysis increased pH significantly to unsuitable levels for methanogenic growth, while NH4Cl had a negligible effect on pH. After adjusting initial pH to 7 and 8, urea was significantly stronger inhibitor with longer lag phases to methanogenesis compared to NH4Cl. Overall, urea seems to be more toxic on both aceticlastic and hydrogenotrophic methanogens compared to NH4Cl under the same total and free ammonia levels.

KW - Ammonia inhibition

KW - Ammonium chloride

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KW - Pure strain

KW - Urea

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Effect of different ammonia sources on aceticlastic and hydrogenotrophic methanogens

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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