Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation

Nazely Diban; Suvi P. Haimi; Lydia Bolhuis-Versteeg; Sandra Teixeira; Susanna Miettinen; André A. Poot; Dirk W. Grijpma; Dimitrios Stamatialis

doi:10.1002/masy.201300106

Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation

Nazely Diban, Suvi P. Haimi, Lydia Bolhuis-Versteeg, Sandra Teixeira, Susanna Miettinen, André A. Poot, Dirk W. Grijpma, Dimitrios Stamatialis

Research output: Contribution to journal › Article › Scientific › peer-review

5 Citations (Scopus)

Abstract

Summary The effect of the surface morphology of flat poly(Ïμ- caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.

Original language	English
Pages (from-to)	126-132
Number of pages	7
Journal	Macromolecular symposia
Volume	334
Issue number	1
DOIs	https://doi.org/10.1002/masy.201300106
Publication status	Published - Dec 2013
Publication type	A1 Journal article-refereed

Keywords

morphology
phase inversion
poly(Ïμ-caprolactone)
roughness
scaffold porosity

ASJC Scopus subject areas

Organic Chemistry
Materials Chemistry
Polymers and Plastics
Condensed Matter Physics

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10.1002/masy.201300106

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title = "Effect of surface morphology of poly({\"i}μ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation",

abstract = "Summary The effect of the surface morphology of flat poly({\"I}μ- caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.",

keywords = "morphology, phase inversion, poly({\"I}μ-caprolactone), roughness, scaffold porosity",

author = "Nazely Diban and Haimi, {Suvi P.} and Lydia Bolhuis-Versteeg and Sandra Teixeira and Susanna Miettinen and Poot, {Andr{\'e} A.} and Grijpma, {Dirk W.} and Dimitrios Stamatialis",

year = "2013",

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doi = "10.1002/masy.201300106",

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T1 - Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation

AU - Diban, Nazely

AU - Haimi, Suvi P.

AU - Bolhuis-Versteeg, Lydia

AU - Teixeira, Sandra

AU - Miettinen, Susanna

AU - Poot, André A.

AU - Grijpma, Dirk W.

AU - Stamatialis, Dimitrios

PY - 2013/12

Y1 - 2013/12

N2 - Summary The effect of the surface morphology of flat poly(Ïμ- caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.

AB - Summary The effect of the surface morphology of flat poly(Ïμ- caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.

KW - morphology

KW - phase inversion

KW - poly(Ïμ-caprolactone)

KW - roughness

KW - scaffold porosity

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DO - 10.1002/masy.201300106

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ER -

Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation

Abstract

Keywords

ASJC Scopus subject areas

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