Effect of the type and concentration of cellulose and temperature on metabolite formation by a fermentative thermophilic consortium

Sarah M. Carver; Raghida Lepistö; Olli H. Tuovinen

doi:10.1016/j.ijhydene.2019.02.177

Effect of the type and concentration of cellulose and temperature on metabolite formation by a fermentative thermophilic consortium

Sarah M. Carver
, Raghida Lepistö
, Olli H. Tuovinen^*

^*Corresponding author for this work

Materials Science and Environmental Engineering

Research output: Contribution to journal › Article › Scientific › peer-review

5 Citations (Scopus)

Abstract

In this study, we hypothesized that anaerobic biodegradation of cellulose is influenced by cellulose type and concentration, temperature, and their interactions. Cellulose biodegradation by an anaerobic consortium was tested in thermophilic batch experiments that combined cellulase action, hydrolysis, and fermentation. Initially, the main constituents in the inocula were Thermoanaerobacter, Clostridium, and Acetivibrio spp. Four types of cellulose and a range of concentrations were used as feedstock with pathways involving hydrolysis and glycolysis to produce H₂, CO₂, acetate, and ethanol. Long fibrous cellulose, two types of microcrystalline cellulose, and filter paper squares were tested at several concentrations between 2 and 20 g/l as substrates. The yields ranged between 0.1 and 2.9 mmol H₂ and 0.7–2.6 mmol ethanol per g cellulose. The rates ranged between 0.01 and 0.2 mmol H₂, 0.03–0.2 mmol CO₂, and 0.01–0.05 mmol ethanol per g cellulose·h. Statistical analyses indicated that the rates and yields of metabolite production were influenced by two-way interactions between the temperature, type, and concentration of cellulose. The results suggest that two-way interactions between experimental variables may impact the outcomes in cellulose bioconversion studies.

Original language	English
Pages (from-to)	17248-17259
Number of pages	12
Journal	International Journal of Hydrogen Energy
Volume	44
Issue number	32
DOIs	https://doi.org/10.1016/j.ijhydene.2019.02.177
Publication status	Published - 28 Jun 2019
Publication type	A1 Journal article-refereed

Funding

We thank the Statistical Consulting Service team (Department of Statistics, Ohio State University) for providing assistance with the ANOVA and Tukey methods. F.S. Jones and J.M. Bigham (School of Environmental and Natural Resources, OSU) were instrumental in XRD analysis and crystallinity index determination. This work was part of a project supported by the Finnish Agency of Technology and Innovation (Finland Distinguished Professor Program 402/2006). The study was also funded, in part, by the United States Department of Agriculture National Needs Graduate Fellowship Program through the National Institute of Food and Agriculture under Agreement No. 2005-38420-15792 (S.M.C.). We thank the anonymous reviewers for insightful suggestions that were helpful in improving the manuscript.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Cellulosic substrate
Clostridia
Ethanol production
Fermentation
Hydrogen production

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

Access to Document

10.1016/j.ijhydene.2019.02.177

Cite this

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title = "Effect of the type and concentration of cellulose and temperature on metabolite formation by a fermentative thermophilic consortium",

abstract = "In this study, we hypothesized that anaerobic biodegradation of cellulose is influenced by cellulose type and concentration, temperature, and their interactions. Cellulose biodegradation by an anaerobic consortium was tested in thermophilic batch experiments that combined cellulase action, hydrolysis, and fermentation. Initially, the main constituents in the inocula were Thermoanaerobacter, Clostridium, and Acetivibrio spp. Four types of cellulose and a range of concentrations were used as feedstock with pathways involving hydrolysis and glycolysis to produce H2, CO2, acetate, and ethanol. Long fibrous cellulose, two types of microcrystalline cellulose, and filter paper squares were tested at several concentrations between 2 and 20 g/l as substrates. The yields ranged between 0.1 and 2.9 mmol H2 and 0.7–2.6 mmol ethanol per g cellulose. The rates ranged between 0.01 and 0.2 mmol H2, 0.03–0.2 mmol CO2, and 0.01–0.05 mmol ethanol per g cellulose·h. Statistical analyses indicated that the rates and yields of metabolite production were influenced by two-way interactions between the temperature, type, and concentration of cellulose. The results suggest that two-way interactions between experimental variables may impact the outcomes in cellulose bioconversion studies.",

keywords = "Cellulosic substrate, Clostridia, Ethanol production, Fermentation, Hydrogen production",

author = "Carver, \{Sarah M.\} and Raghida Lepist{\"o} and Tuovinen, \{Olli H.\}",

note = "Funding Information: We thank the Statistical Consulting Service team (Department of Statistics, Ohio State University) for providing assistance with the ANOVA and Tukey methods. F.S. Jones and J.M. Bigham (School of Environmental and Natural Resources, OSU) were instrumental in XRD analysis and crystallinity index determination. This work was part of a project supported by the Finnish Agency of Technology and Innovation (Finland Distinguished Professor Program 402/2006). The study was also funded, in part, by the United States Department of Agriculture National Needs Graduate Fellowship Program through the National Institute of Food and Agriculture under Agreement No. 2005-38420-15792 (S.M.C.). We thank the anonymous reviewers for insightful suggestions that were helpful in improving the manuscript. Publisher Copyright: {\textcopyright} 2019 Hydrogen Energy Publications LLC Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

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doi = "10.1016/j.ijhydene.2019.02.177",

language = "English",

volume = "44",

pages = "17248--17259",

journal = "International Journal of Hydrogen Energy",

issn = "0360-3199",

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T1 - Effect of the type and concentration of cellulose and temperature on metabolite formation by a fermentative thermophilic consortium

AU - Carver, Sarah M.

AU - Lepistö, Raghida

AU - Tuovinen, Olli H.

N1 - Funding Information: We thank the Statistical Consulting Service team (Department of Statistics, Ohio State University) for providing assistance with the ANOVA and Tukey methods. F.S. Jones and J.M. Bigham (School of Environmental and Natural Resources, OSU) were instrumental in XRD analysis and crystallinity index determination. This work was part of a project supported by the Finnish Agency of Technology and Innovation (Finland Distinguished Professor Program 402/2006). The study was also funded, in part, by the United States Department of Agriculture National Needs Graduate Fellowship Program through the National Institute of Food and Agriculture under Agreement No. 2005-38420-15792 (S.M.C.). We thank the anonymous reviewers for insightful suggestions that were helpful in improving the manuscript. Publisher Copyright: © 2019 Hydrogen Energy Publications LLC Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/6/28

Y1 - 2019/6/28

N2 - In this study, we hypothesized that anaerobic biodegradation of cellulose is influenced by cellulose type and concentration, temperature, and their interactions. Cellulose biodegradation by an anaerobic consortium was tested in thermophilic batch experiments that combined cellulase action, hydrolysis, and fermentation. Initially, the main constituents in the inocula were Thermoanaerobacter, Clostridium, and Acetivibrio spp. Four types of cellulose and a range of concentrations were used as feedstock with pathways involving hydrolysis and glycolysis to produce H2, CO2, acetate, and ethanol. Long fibrous cellulose, two types of microcrystalline cellulose, and filter paper squares were tested at several concentrations between 2 and 20 g/l as substrates. The yields ranged between 0.1 and 2.9 mmol H2 and 0.7–2.6 mmol ethanol per g cellulose. The rates ranged between 0.01 and 0.2 mmol H2, 0.03–0.2 mmol CO2, and 0.01–0.05 mmol ethanol per g cellulose·h. Statistical analyses indicated that the rates and yields of metabolite production were influenced by two-way interactions between the temperature, type, and concentration of cellulose. The results suggest that two-way interactions between experimental variables may impact the outcomes in cellulose bioconversion studies.

AB - In this study, we hypothesized that anaerobic biodegradation of cellulose is influenced by cellulose type and concentration, temperature, and their interactions. Cellulose biodegradation by an anaerobic consortium was tested in thermophilic batch experiments that combined cellulase action, hydrolysis, and fermentation. Initially, the main constituents in the inocula were Thermoanaerobacter, Clostridium, and Acetivibrio spp. Four types of cellulose and a range of concentrations were used as feedstock with pathways involving hydrolysis and glycolysis to produce H2, CO2, acetate, and ethanol. Long fibrous cellulose, two types of microcrystalline cellulose, and filter paper squares were tested at several concentrations between 2 and 20 g/l as substrates. The yields ranged between 0.1 and 2.9 mmol H2 and 0.7–2.6 mmol ethanol per g cellulose. The rates ranged between 0.01 and 0.2 mmol H2, 0.03–0.2 mmol CO2, and 0.01–0.05 mmol ethanol per g cellulose·h. Statistical analyses indicated that the rates and yields of metabolite production were influenced by two-way interactions between the temperature, type, and concentration of cellulose. The results suggest that two-way interactions between experimental variables may impact the outcomes in cellulose bioconversion studies.

KW - Cellulosic substrate

KW - Clostridia

KW - Ethanol production

KW - Fermentation

KW - Hydrogen production

U2 - 10.1016/j.ijhydene.2019.02.177

DO - 10.1016/j.ijhydene.2019.02.177

M3 - Article

AN - SCOPUS:85063100473

SN - 0360-3199

VL - 44

SP - 17248

EP - 17259

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

IS - 32

ER -

Effect of the type and concentration of cellulose and temperature on metabolite formation by a fermentative thermophilic consortium

Abstract

Funding

UN SDGs

Keywords

Publication forum classification

ASJC Scopus subject areas

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Cite this