TY - JOUR
T1 - Effects of bioactive glass S53P4 or beta-tricalcium phosphate and bone morphogenetic protein-2 and bone morphogenetic protein-7 on osteogenic differentiation of human adipose stem cells
AU - Waselau, Martin
AU - Patrikoski, Mimmi
AU - Juntunen, Miia
AU - Kujala, Kasperi
AU - Kääriäinen, Minna
AU - Kuokkanen, Hannu
AU - Sandor, George
AU - Vapaavuori, Outi
AU - Suuronen, Riitta
AU - Mannerström, Bettina
AU - von Rechenberg, Brigitte
AU - Miettinen, Susanna
AU - von RECHENBERG, B
PY - 2012
Y1 - 2012
N2 - The effects of bioactive glass S53P4 or beta-tricalcium phosphate: and bone morphogenetic proteins bone morphogenetic protein-2, bone morphogenetic protein-7, or bone morphogenetic protein-2 + 7 on osteogenic differentiation of human adipose stem cells were compared in control medium, osteogenic medium, and bone morphogenetic proteinsupplemented osteogenic medium to assess suitability for bone tissue engineering. Cell amount was evaluated with qDNA measurements: osteogenic differentiation using marker gene expression, alkaline phosphate activity, and angiogenic potential was measured by vascular endothelial growth factor expression. As compared to beta-tricalcium phosphate, cell amount was significantly greater for bioactive glass in control medium after 7 days and in osteogenic medium after 14 days, and alkaline phosphate activity was always significantly greater for bioactive glass in control medium. However, alkaline phosphate activity increased for beta-tricalcium phosphate and decreased for bioactive glass granules in osteogenic medium. For both biomaterials, bone morphogenetic protein supplementation decreased cell amount and osteogenic differentiation of human adipose stem cells, and vascular endothelial growth factor expressions correlated with cell amounts. Effects of culture medium on human adipose stem cells are biomaterial dependent: bioactive glass in control medium enhanced osteogenic differentiation most effectively.
AB - The effects of bioactive glass S53P4 or beta-tricalcium phosphate: and bone morphogenetic proteins bone morphogenetic protein-2, bone morphogenetic protein-7, or bone morphogenetic protein-2 + 7 on osteogenic differentiation of human adipose stem cells were compared in control medium, osteogenic medium, and bone morphogenetic proteinsupplemented osteogenic medium to assess suitability for bone tissue engineering. Cell amount was evaluated with qDNA measurements: osteogenic differentiation using marker gene expression, alkaline phosphate activity, and angiogenic potential was measured by vascular endothelial growth factor expression. As compared to beta-tricalcium phosphate, cell amount was significantly greater for bioactive glass in control medium after 7 days and in osteogenic medium after 14 days, and alkaline phosphate activity was always significantly greater for bioactive glass in control medium. However, alkaline phosphate activity increased for beta-tricalcium phosphate and decreased for bioactive glass granules in osteogenic medium. For both biomaterials, bone morphogenetic protein supplementation decreased cell amount and osteogenic differentiation of human adipose stem cells, and vascular endothelial growth factor expressions correlated with cell amounts. Effects of culture medium on human adipose stem cells are biomaterial dependent: bioactive glass in control medium enhanced osteogenic differentiation most effectively.
KW - Beta-tricalcium phosphate
KW - Bioactive glass
KW - Bone morphogenetic protein-2
KW - Bone morphogenetic protein-7
KW - Human adipose stem cells
KW - In vitro
KW - Osteogenic differentiation
KW - Beta-tricalcium phosphate
KW - Bioactive glass
KW - Bone morphogenetic protein-2
KW - Bone morphogenetic protein-7
KW - Human adipose stem cells
KW - In vitro
KW - Osteogenic differentiation
U2 - 10.1177/2041731412467789
DO - 10.1177/2041731412467789
M3 - Article
AN - SCOPUS:84888424310
SN - 2041-7314
VL - 3
SP - 1
EP - 14
JO - Journal of Tissue Engineering
JF - Journal of Tissue Engineering
IS - 1
ER -