Effects of bioactive glass S53P4 or beta-tricalcium phosphate and bone morphogenetic protein-2 and bone morphogenetic protein-7 on osteogenic differentiation of human adipose stem cells

Martin Waselau, Mimmi Patrikoski, Miia Juntunen, Kasperi Kujala, Minna Kääriäinen, Hannu Kuokkanen, George Sandor, Outi Vapaavuori, Riitta Suuronen, Bettina Mannerström, Brigitte von Rechenberg, Susanna Miettinen, B von RECHENBERG

    Research output: Contribution to journalArticleScientificpeer-review

    17 Citations (Scopus)

    Abstract

    The effects of bioactive glass S53P4 or beta-tricalcium phosphate: and bone morphogenetic proteins bone morphogenetic protein-2, bone morphogenetic protein-7, or bone morphogenetic protein-2 + 7 on osteogenic differentiation of human adipose stem cells were compared in control medium, osteogenic medium, and bone morphogenetic proteinsupplemented osteogenic medium to assess suitability for bone tissue engineering. Cell amount was evaluated with qDNA measurements: osteogenic differentiation using marker gene expression, alkaline phosphate activity, and angiogenic potential was measured by vascular endothelial growth factor expression. As compared to beta-tricalcium phosphate, cell amount was significantly greater for bioactive glass in control medium after 7 days and in osteogenic medium after 14 days, and alkaline phosphate activity was always significantly greater for bioactive glass in control medium. However, alkaline phosphate activity increased for beta-tricalcium phosphate and decreased for bioactive glass granules in osteogenic medium. For both biomaterials, bone morphogenetic protein supplementation decreased cell amount and osteogenic differentiation of human adipose stem cells, and vascular endothelial growth factor expressions correlated with cell amounts. Effects of culture medium on human adipose stem cells are biomaterial dependent: bioactive glass in control medium enhanced osteogenic differentiation most effectively.

    Original languageEnglish
    Pages (from-to)1-14
    Number of pages14
    JournalJournal of Tissue Engineering
    Volume3
    Issue number1
    DOIs
    Publication statusPublished - 2012
    Publication typeA1 Journal article-refereed

    Keywords

    • Beta-tricalcium phosphate
    • Bioactive glass
    • Bone morphogenetic protein-2
    • Bone morphogenetic protein-7
    • Human adipose stem cells
    • In vitro
    • Osteogenic differentiation

    Publication forum classification

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