Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats

I Pörsti; H Wuorela; P Arvola; P Säynävälammi; A K Nurmi; H Huhtala; P Laippala; T Metsä-Ketelä; H Vapaatalo

doi:10.3109/10641969009074746

Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats

I Pörsti, H Wuorela, P Arvola, P Säynävälammi, A K Nurmi, H Huhtala, P Laippala, T Metsä-Ketelä, H Vapaatalo

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

Abstract

The effects of calcium and deoxycorticosterone (DOC) on blood pressure, plasma renin activity (PRA), urinary sodium excretion and aortic responses were studied in spontaneously hypertensive rats (SHR). The animals (age 9 weeks) were divided into four treatment groups: control, calcium, DOC and DOC+calcium (n = 12 and the mean systolic blood pressure 174-177 mmHg in each). Calcium was given as 1.5% CaCl2 in drinking fluid, and DOC trimethylacetate by weekly injections (25 mg/kg s.c.). During the 4-week study systolic blood pressure rose in all groups, but the increase was attenuated by calcium (final levels: control 201 +/- 3, calcium 186 +/- 3, DOC 206 +/- 2, DOC + calcium 203 +/- 2 mmHg, mean +/- SE). PRA was reduced in both groups receiving DOC, but it was not affected by calcium. Calcium supplementation increased urinary excretion of sodium in DOC-treated animals. DOC enhanced the in vitro contractility of helically cut aortic strips to noradrenaline, and decreased the relaxation of the strips to nitroprusside and nifedipine. The results indicate that calcium supplementation attenuates the development of hypertension in SHR, and that this attenuation is not mediated by the renin-angiotensin system. DOC abolished this lowering effect of calcium on blood pressure possibly by its action on vascular smooth muscle, resulting in increased vascular contractility and impaired relaxation.

Original language	English
Pages (from-to)	1159-74
Number of pages	16
Journal	Clinical and experimental hypertension. Part A, Theory and practice
Volume	12
Issue number	7
DOIs	https://doi.org/10.3109/10641969009074746
Publication status	Published - 1990
Publication type	A1 Journal article-refereed

Keywords

Animals
Blood Pressure/drug effects
Blood Vessels/drug effects
Body Weight/drug effects
Calcium/pharmacology
Desoxycorticosterone/pharmacology
Diuresis/drug effects
Dose-Response Relationship, Drug
Male
Nifedipine/pharmacology
Nitroprusside/pharmacology
Norepinephrine/pharmacology
Rats
Rats, Inbred SHR
Renin/blood
Sodium/urine
Vasoconstriction/drug effects

Access to Document

10.3109/10641969009074746

Cite this

Pörsti, I., Wuorela, H., Arvola, P., Säynävälammi, P., Nurmi, A. K., Huhtala, H., Laippala, P., Metsä-Ketelä, T., & Vapaatalo, H. (1990). Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats. Clinical and experimental hypertension. Part A, Theory and practice, 12(7), 1159-74. https://doi.org/10.3109/10641969009074746

@article{c8e2dccfd1fa417cab19fbc73195bae9,

title = "Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats",

abstract = "The effects of calcium and deoxycorticosterone (DOC) on blood pressure, plasma renin activity (PRA), urinary sodium excretion and aortic responses were studied in spontaneously hypertensive rats (SHR). The animals (age 9 weeks) were divided into four treatment groups: control, calcium, DOC and DOC+calcium (n = 12 and the mean systolic blood pressure 174-177 mmHg in each). Calcium was given as 1.5% CaCl2 in drinking fluid, and DOC trimethylacetate by weekly injections (25 mg/kg s.c.). During the 4-week study systolic blood pressure rose in all groups, but the increase was attenuated by calcium (final levels: control 201 +/- 3, calcium 186 +/- 3, DOC 206 +/- 2, DOC + calcium 203 +/- 2 mmHg, mean +/- SE). PRA was reduced in both groups receiving DOC, but it was not affected by calcium. Calcium supplementation increased urinary excretion of sodium in DOC-treated animals. DOC enhanced the in vitro contractility of helically cut aortic strips to noradrenaline, and decreased the relaxation of the strips to nitroprusside and nifedipine. The results indicate that calcium supplementation attenuates the development of hypertension in SHR, and that this attenuation is not mediated by the renin-angiotensin system. DOC abolished this lowering effect of calcium on blood pressure possibly by its action on vascular smooth muscle, resulting in increased vascular contractility and impaired relaxation.",

keywords = "Animals, Blood Pressure/drug effects, Blood Vessels/drug effects, Body Weight/drug effects, Calcium/pharmacology, Desoxycorticosterone/pharmacology, Diuresis/drug effects, Dose-Response Relationship, Drug, Male, Nifedipine/pharmacology, Nitroprusside/pharmacology, Norepinephrine/pharmacology, Rats, Rats, Inbred SHR, Renin/blood, Sodium/urine, Vasoconstriction/drug effects",

author = "I P{\"o}rsti and H Wuorela and P Arvola and P S{\"a}yn{\"a}v{\"a}lammi and Nurmi, {A K} and H Huhtala and P Laippala and T Mets{\"a}-Ketel{\"a} and H Vapaatalo",

year = "1990",

doi = "10.3109/10641969009074746",

language = "English",

volume = "12",

pages = "1159--74",

number = "7",

}

Pörsti, I, Wuorela, H, Arvola, P, Säynävälammi, P, Nurmi, AK, Huhtala, H, Laippala, P, Metsä-Ketelä, T & Vapaatalo, H 1990, 'Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats', Clinical and experimental hypertension. Part A, Theory and practice, vol. 12, no. 7, pp. 1159-74. https://doi.org/10.3109/10641969009074746

Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats. / Pörsti, I; Wuorela, H; Arvola, P et al.
In: Clinical and experimental hypertension. Part A, Theory and practice, Vol. 12, No. 7, 1990, p. 1159-74.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats

AU - Pörsti, I

AU - Wuorela, H

AU - Arvola, P

AU - Säynävälammi, P

AU - Nurmi, A K

AU - Huhtala, H

AU - Laippala, P

AU - Metsä-Ketelä, T

AU - Vapaatalo, H

PY - 1990

Y1 - 1990

N2 - The effects of calcium and deoxycorticosterone (DOC) on blood pressure, plasma renin activity (PRA), urinary sodium excretion and aortic responses were studied in spontaneously hypertensive rats (SHR). The animals (age 9 weeks) were divided into four treatment groups: control, calcium, DOC and DOC+calcium (n = 12 and the mean systolic blood pressure 174-177 mmHg in each). Calcium was given as 1.5% CaCl2 in drinking fluid, and DOC trimethylacetate by weekly injections (25 mg/kg s.c.). During the 4-week study systolic blood pressure rose in all groups, but the increase was attenuated by calcium (final levels: control 201 +/- 3, calcium 186 +/- 3, DOC 206 +/- 2, DOC + calcium 203 +/- 2 mmHg, mean +/- SE). PRA was reduced in both groups receiving DOC, but it was not affected by calcium. Calcium supplementation increased urinary excretion of sodium in DOC-treated animals. DOC enhanced the in vitro contractility of helically cut aortic strips to noradrenaline, and decreased the relaxation of the strips to nitroprusside and nifedipine. The results indicate that calcium supplementation attenuates the development of hypertension in SHR, and that this attenuation is not mediated by the renin-angiotensin system. DOC abolished this lowering effect of calcium on blood pressure possibly by its action on vascular smooth muscle, resulting in increased vascular contractility and impaired relaxation.

AB - The effects of calcium and deoxycorticosterone (DOC) on blood pressure, plasma renin activity (PRA), urinary sodium excretion and aortic responses were studied in spontaneously hypertensive rats (SHR). The animals (age 9 weeks) were divided into four treatment groups: control, calcium, DOC and DOC+calcium (n = 12 and the mean systolic blood pressure 174-177 mmHg in each). Calcium was given as 1.5% CaCl2 in drinking fluid, and DOC trimethylacetate by weekly injections (25 mg/kg s.c.). During the 4-week study systolic blood pressure rose in all groups, but the increase was attenuated by calcium (final levels: control 201 +/- 3, calcium 186 +/- 3, DOC 206 +/- 2, DOC + calcium 203 +/- 2 mmHg, mean +/- SE). PRA was reduced in both groups receiving DOC, but it was not affected by calcium. Calcium supplementation increased urinary excretion of sodium in DOC-treated animals. DOC enhanced the in vitro contractility of helically cut aortic strips to noradrenaline, and decreased the relaxation of the strips to nitroprusside and nifedipine. The results indicate that calcium supplementation attenuates the development of hypertension in SHR, and that this attenuation is not mediated by the renin-angiotensin system. DOC abolished this lowering effect of calcium on blood pressure possibly by its action on vascular smooth muscle, resulting in increased vascular contractility and impaired relaxation.

KW - Animals

KW - Blood Pressure/drug effects

KW - Blood Vessels/drug effects

KW - Body Weight/drug effects

KW - Calcium/pharmacology

KW - Desoxycorticosterone/pharmacology

KW - Diuresis/drug effects

KW - Dose-Response Relationship, Drug

KW - Male

KW - Nifedipine/pharmacology

KW - Nitroprusside/pharmacology

KW - Norepinephrine/pharmacology

KW - Rats

KW - Rats, Inbred SHR

KW - Renin/blood

KW - Sodium/urine

KW - Vasoconstriction/drug effects

U2 - 10.3109/10641969009074746

DO - 10.3109/10641969009074746

M3 - Article

C2 - 2257685

SN - 0730-0077

VL - 12

SP - 1159

EP - 1174

JO - Clinical and experimental hypertension. Part A, Theory and practice

JF - Clinical and experimental hypertension. Part A, Theory and practice

IS - 7

ER -

Effects of calcium and deoxycorticosterone on blood pressure, plasma renin activity and vascular reactivity in spontaneously hypertensive rats

Abstract

Keywords

Access to Document

Fingerprint

Cite this