Effects of transcriptional pausing on gene expression dynamics

Tiina Rajala, Antti Häkkinen, Shannon Healy, Olli Yli-Harja, Andre S. Ribeiro

    Research output: Contribution to journalArticleScientificpeer-review

    35 Citations (Scopus)
    5 Downloads (Pure)

    Abstract

    Stochasticity in gene expression affects many cellular processes and is a source of phenotypic diversity between genetically identical individuals. Events in elongation, particularly RNA polymerase pausing, are a source of this noise. Since the rate and duration of pausing are sequence-dependent, this regulatory mechanism of transcriptional dynamics is evolvable. The dependency of pause propensity on regulatory molecules makes pausing a response mechanism to external stress. Using a delayed stochastic model of bacterial transcription at the single nucleotide level that includes the promoter open complex formation, pausing, arrest, misincorporation and editing, pyrophosphorolysis, and premature termination, we investigate how RNA polymerase pausing affects a gene's transcriptional dynamics and gene networks. We show that pauses' duration and rate of occurrence affect the bursting in RNA production, transcriptional and translational noise, and the transient to reach mean RNA and protein levels. In a genetic repressilator, increasing the pausing rate and the duration of pausing events increases the period length but does not affect the robustness of the periodicity. We conclude that RNA polymerase pausing might be an important evolvable feature of genetic networks.
    Translated title of the contributionEffects of transcriptional pausing on gene expression dynamics
    Original languageEnglish
    Pages (from-to)1-12
    Number of pages12
    JournalPLoS Computational Biology
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 2010
    Publication typeA1 Journal article-refereed

    Publication forum classification

    • Publication forum level 3

    Fingerprint

    Dive into the research topics of 'Effects of transcriptional pausing on gene expression dynamics'. Together they form a unique fingerprint.

    Cite this