Effects of triclosan, diclofenac, and nonylphenol on mesophilic and thermophilic methanogenic activity and on the methanogenic communities

Evangelos C. Symsaris; Ioannis A. Fotidis; Athanasios S. Stasinakis; Irini Angelidaki

doi:10.1016/j.jhazmat.2015.03.002

Effects of triclosan, diclofenac, and nonylphenol on mesophilic and thermophilic methanogenic activity and on the methanogenic communities

Evangelos C. Symsaris
, Ioannis A. Fotidis
, Athanasios S. Stasinakis
, Irini Angelidaki^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

41 Citations (Scopus)

Abstract

In this study, a toxicity assay using a mesophilic wastewater treatment plant sludge-based (SI) and a thermophilic manure-based inoculum (MI), under different biomass concentrations was performed to define the effects of diclofenac (DCF), triclosan (TCS), and nonylphenol (NP) on anaerobic digestion (AD) process. Additionally, the influence of DCF, TCS, and NP on the relative abundance of the methanogenic populations was investigated. Results obtained demonstrated that, in terms of methane production, SI inoculum was more resistant to the toxicity effect of DCF, TCS, and NP, compared to the MI inoculum. The IC₅₀ values were 546, 35, and 363mgL^-1 for SI inoculum and 481, 32, and 74mgL^-1 for MI inoculum for DCF, TCS, and NP, respectively. For both inocula, higher biomass concentrations reduced the toxic effect of TCS (higher methane production up to 64%), contrary to DCF, where higher biomass loads decreased methane yield up to 31%. Fluorescence in situ hybridization analysis showed that hydrogenotrophic methanogens were more resistant to the inhibitory effect of DCF, TCS, and NP compared to aceticlastic methanogens.

Original language	English
Pages (from-to)	45-51
Number of pages	7
Journal	Journal of Hazardous Materials
Volume	291
DOIs	https://doi.org/10.1016/j.jhazmat.2015.03.002
Publication status	Published - 2015
Externally published	Yes
Publication type	A1 Journal article-refereed

Funding

We thank Hector Garcia for technical assistance with the experiment. This research has been co-financed by the European Union (European Social Fund – ESF) and Greek National Funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) – Research Funding Program: THALES. Invesment in knowledge society through the European Social Fund. WATERMICROPOL: www.aegean.gr/environment/watermicropol .

Keywords

Biomethanation
IC
PPCPs
Toxicity
Xenobiotics

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution
Health, Toxicology and Mutagenesis

Access to Document

10.1016/j.jhazmat.2015.03.002

Cite this

@article{6d344633e95f454490f4be916a8561f3,

title = "Effects of triclosan, diclofenac, and nonylphenol on mesophilic and thermophilic methanogenic activity and on the methanogenic communities",

abstract = "In this study, a toxicity assay using a mesophilic wastewater treatment plant sludge-based (SI) and a thermophilic manure-based inoculum (MI), under different biomass concentrations was performed to define the effects of diclofenac (DCF), triclosan (TCS), and nonylphenol (NP) on anaerobic digestion (AD) process. Additionally, the influence of DCF, TCS, and NP on the relative abundance of the methanogenic populations was investigated. Results obtained demonstrated that, in terms of methane production, SI inoculum was more resistant to the toxicity effect of DCF, TCS, and NP, compared to the MI inoculum. The IC50 values were 546, 35, and 363mgL-1 for SI inoculum and 481, 32, and 74mgL-1 for MI inoculum for DCF, TCS, and NP, respectively. For both inocula, higher biomass concentrations reduced the toxic effect of TCS (higher methane production up to 64\%), contrary to DCF, where higher biomass loads decreased methane yield up to 31\%. Fluorescence in situ hybridization analysis showed that hydrogenotrophic methanogens were more resistant to the inhibitory effect of DCF, TCS, and NP compared to aceticlastic methanogens.",

keywords = "Biomethanation, IC, PPCPs, Toxicity, Xenobiotics",

author = "Symsaris, \{Evangelos C.\} and Fotidis, \{Ioannis A.\} and Stasinakis, \{Athanasios S.\} and Irini Angelidaki",

note = "Funding Information: We thank Hector Garcia for technical assistance with the experiment. This research has been co-financed by the European Union (European Social Fund – ESF) and Greek National Funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) – Research Funding Program: THALES. Invesment in knowledge society through the European Social Fund. WATERMICROPOL: www.aegean.gr/environment/watermicropol . Publisher Copyright: {\textcopyright} 2015 Elsevier B.V. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "2015",

doi = "10.1016/j.jhazmat.2015.03.002",

language = "English",

volume = "291",

pages = "45--51",

journal = "Journal of Hazardous Materials",

issn = "0304-3894",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Effects of triclosan, diclofenac, and nonylphenol on mesophilic and thermophilic methanogenic activity and on the methanogenic communities

AU - Symsaris, Evangelos C.

AU - Fotidis, Ioannis A.

AU - Stasinakis, Athanasios S.

AU - Angelidaki, Irini

N1 - Funding Information: We thank Hector Garcia for technical assistance with the experiment. This research has been co-financed by the European Union (European Social Fund – ESF) and Greek National Funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) – Research Funding Program: THALES. Invesment in knowledge society through the European Social Fund. WATERMICROPOL: www.aegean.gr/environment/watermicropol . Publisher Copyright: © 2015 Elsevier B.V. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 2015

Y1 - 2015

N2 - In this study, a toxicity assay using a mesophilic wastewater treatment plant sludge-based (SI) and a thermophilic manure-based inoculum (MI), under different biomass concentrations was performed to define the effects of diclofenac (DCF), triclosan (TCS), and nonylphenol (NP) on anaerobic digestion (AD) process. Additionally, the influence of DCF, TCS, and NP on the relative abundance of the methanogenic populations was investigated. Results obtained demonstrated that, in terms of methane production, SI inoculum was more resistant to the toxicity effect of DCF, TCS, and NP, compared to the MI inoculum. The IC50 values were 546, 35, and 363mgL-1 for SI inoculum and 481, 32, and 74mgL-1 for MI inoculum for DCF, TCS, and NP, respectively. For both inocula, higher biomass concentrations reduced the toxic effect of TCS (higher methane production up to 64%), contrary to DCF, where higher biomass loads decreased methane yield up to 31%. Fluorescence in situ hybridization analysis showed that hydrogenotrophic methanogens were more resistant to the inhibitory effect of DCF, TCS, and NP compared to aceticlastic methanogens.

AB - In this study, a toxicity assay using a mesophilic wastewater treatment plant sludge-based (SI) and a thermophilic manure-based inoculum (MI), under different biomass concentrations was performed to define the effects of diclofenac (DCF), triclosan (TCS), and nonylphenol (NP) on anaerobic digestion (AD) process. Additionally, the influence of DCF, TCS, and NP on the relative abundance of the methanogenic populations was investigated. Results obtained demonstrated that, in terms of methane production, SI inoculum was more resistant to the toxicity effect of DCF, TCS, and NP, compared to the MI inoculum. The IC50 values were 546, 35, and 363mgL-1 for SI inoculum and 481, 32, and 74mgL-1 for MI inoculum for DCF, TCS, and NP, respectively. For both inocula, higher biomass concentrations reduced the toxic effect of TCS (higher methane production up to 64%), contrary to DCF, where higher biomass loads decreased methane yield up to 31%. Fluorescence in situ hybridization analysis showed that hydrogenotrophic methanogens were more resistant to the inhibitory effect of DCF, TCS, and NP compared to aceticlastic methanogens.

KW - Biomethanation

KW - IC

KW - PPCPs

KW - Toxicity

KW - Xenobiotics

U2 - 10.1016/j.jhazmat.2015.03.002

DO - 10.1016/j.jhazmat.2015.03.002

M3 - Article

C2 - 25768988

AN - SCOPUS:84924322652

SN - 0304-3894

VL - 291

SP - 45

EP - 51

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

ER -

Effects of triclosan, diclofenac, and nonylphenol on mesophilic and thermophilic methanogenic activity and on the methanogenic communities

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this