Conversation analytical studies on emotion show how expression of emotion is part of the intersubjective experience. Emotions, however, are as much physiological as experiential events. Physiological processes pertaining to emotion involve changes in cardiovascular activity, in the activation of sweat glands, and in muscular activity. The dyadic systems theory by Beebe and Lachmann (2002) suggests that actions that regulate social interaction also serve in the regulation of internal emotional states of interacting subjects. Drawing from this theory, our overall research questions was: how is the expression of emotion in social interaction linked to physiological responses in the participants? Our main result was that thorough conversational affiliation, the participants share the emotional load in the interaction.