Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1

Changshuo Liu, Bohyun Choi, Elena Efimova, Yvonne Nygård, Suvi Santala

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Background: Lignocellulosic biomass as feedstock has a huge potential for biochemical production. Still, efficient utilization of hydrolysates derived from lignocellulose is challenged by their complex and heterogeneous composition and the presence of inhibitory compounds, such as furan aldehydes. Using microbial consortia where two specialized microbes complement each other could serve as a potential approach to improve the efficiency of lignocellulosic biomass upgrading. Results: This study describes the simultaneous inhibitor detoxification and production of lactic acid and wax esters from a synthetic lignocellulosic hydrolysate by a defined coculture of engineered Saccharomyces cerevisiae and Acinetobacter baylyi ADP1. A. baylyi ADP1 showed efficient bioconversion of furan aldehydes present in the hydrolysate, namely furfural and 5-hydroxymethylfurfural, and did not compete for substrates with S. cerevisiae, highlighting its potential as a coculture partner. Furthermore, the remaining carbon sources and byproducts of S. cerevisiae were directed to wax ester production by A. baylyi ADP1. The lactic acid productivity of S. cerevisiae was improved approximately 1.5-fold (to 0.41 ± 0.08 g/L/h) in the coculture with A. baylyi ADP1, compared to a monoculture of S. cerevisiae. Conclusion: The coculture of yeast and bacterium was shown to improve the consumption of lignocellulosic substrates and the productivity of lactic acid from a synthetic lignocellulosic hydrolysate. The high detoxification capacity and the ability to produce high-value products by A. baylyi ADP1 demonstrates the strain to be a potential candidate for coculture to increase production efficiency and economics of S. cerevisiae fermentations.

Original languageEnglish
Article number61
JournalBiotechnology for Biofuels and Bioproducts
Volume17
Issue number1
DOIs
Publication statusPublished - 2024
Publication typeA1 Journal article-refereed

Keywords

  • Acinetobacter baylyi ADP1
  • Cocultivation
  • Detoxification
  • Lactic acid
  • Lignocellulose
  • Saccharomyces cerevisiae
  • Wax esters

Publication forum classification

  • Publication forum level 2

ASJC Scopus subject areas

  • Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Applied Microbiology and Biotechnology
  • Energy (miscellaneous)
  • Management, Monitoring, Policy and Law

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