Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser

Gianni Nteroli; Giulia Messa; Stella Koutsikou; Manoj K. Dasa; Antti Penttinen; Antti Härkönen; Mircea Guina; Adrian Podoleanu; Adrian Bradu

doi:10.1117/12.2670901

Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser

Gianni Nteroli, Giulia Messa, Stella Koutsikou, Manoj K. Dasa, Antti Penttinen, Antti Härkönen, Mircea Guina, Adrian Podoleanu, Adrian Bradu

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

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Abstract

In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration <100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~ 3.8 µm lateral resolution throughout the whole axial imaging range.

Original language	English
Title of host publication	Opto-Acoustic Methods and Applications in Biophotonics VI
Editors	Chulhong Kim, Jan Laufer, Vasilis Ntziachristos, Roger J. Zemp
Publisher	SPIE
ISBN (Electronic)	9781510664715
DOIs	https://doi.org/10.1117/12.2670901
Publication status	Published - 2023
Publication type	A4 Article in conference proceedings
Event	Opto-Acoustic Methods and Applications in Biophotonics - Munich, Germany Duration: 25 Jun 2023 → 26 Jun 2023 Conference number: 6 https://spie.org/EBO23/conferencedetails/optoacoustic-methods-and-applications-in-biophotonics

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12631
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Opto-Acoustic Methods and Applications in Biophotonics
Country/Territory	Germany
City	Munich
Period	25/06/23 → 26/06/23
Internet address	https://spie.org/EBO23/conferencedetails/optoacoustic-methods-and-applications-in-biophotonics

Keywords

axial resolution
Optoacoustic Imaging
pico-second lasers

Publication forum classification

Publication forum level 0

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.2670901

Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser
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Nteroli, G., Messa, G., Koutsikou, S., Dasa, M. K., Penttinen, A., Härkönen, A., Guina, M., Podoleanu, A., & Bradu, A. (2023). Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser. In C. Kim, J. Laufer, V. Ntziachristos, & R. J. Zemp (Eds.), Opto-Acoustic Methods and Applications in Biophotonics VI Article 1263106 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12631). SPIE. https://doi.org/10.1117/12.2670901

Nteroli, Gianni ; Messa, Giulia ; Koutsikou, Stella et al. / Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser. Opto-Acoustic Methods and Applications in Biophotonics VI. editor / Chulhong Kim ; Jan Laufer ; Vasilis Ntziachristos ; Roger J. Zemp. SPIE, 2023. (Proceedings of SPIE - The International Society for Optical Engineering).

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title = "Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser",

abstract = "In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration <100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~ 3.8 µm lateral resolution throughout the whole axial imaging range.",

keywords = "axial resolution, Optoacoustic Imaging, pico-second lasers",

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Nteroli, G, Messa, G, Koutsikou, S, Dasa, MK, Penttinen, A, Härkönen, A, Guina, M, Podoleanu, A & Bradu, A 2023, Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser. in C Kim, J Laufer, V Ntziachristos & RJ Zemp (eds), Opto-Acoustic Methods and Applications in Biophotonics VI., 1263106, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12631, SPIE, Opto-Acoustic Methods and Applications in Biophotonics, Munich, Germany, 25/06/23. https://doi.org/10.1117/12.2670901

Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser. / Nteroli, Gianni; Messa, Giulia; Koutsikou, Stella et al.
Opto-Acoustic Methods and Applications in Biophotonics VI. ed. / Chulhong Kim; Jan Laufer; Vasilis Ntziachristos; Roger J. Zemp. SPIE, 2023. 1263106 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12631).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

TY - GEN

T1 - Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser

AU - Nteroli, Gianni

AU - Messa, Giulia

AU - Koutsikou, Stella

AU - Dasa, Manoj K.

AU - Penttinen, Antti

AU - Härkönen, Antti

AU - Guina, Mircea

AU - Podoleanu, Adrian

AU - Bradu, Adrian

N1 - Conference code: 6

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Y1 - 2023

N2 - In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration <100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~ 3.8 µm lateral resolution throughout the whole axial imaging range.

AB - In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration <100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~ 3.8 µm lateral resolution throughout the whole axial imaging range.

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DO - 10.1117/12.2670901

M3 - Conference contribution

AN - SCOPUS:85172860793

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Opto-Acoustic Methods and Applications in Biophotonics VI

A2 - Kim, Chulhong

A2 - Laufer, Jan

A2 - Ntziachristos, Vasilis

A2 - Zemp, Roger J.

PB - SPIE

T2 - Opto-Acoustic Methods and Applications in Biophotonics

Y2 - 25 June 2023 through 26 June 2023

ER -

Nteroli G, Messa G, Koutsikou S, Dasa MK, Penttinen A, Härkönen A et al. Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser. In Kim C, Laufer J, Ntziachristos V, Zemp RJ, editors, Opto-Acoustic Methods and Applications in Biophotonics VI. SPIE. 2023. 1263106. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2670901

Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser

Abstract

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