Enhancing thermophilic dark fermentative hydrogen production at high glucose concentrations via bioaugmentation with Thermotoga neapolitana

Onyinye Okonkwo, Stefano Papirio, Eric Trably, Renaud Escudie, Aino-Maija Lakaniemi, Giovanni Esposito

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
4 Downloads (Pure)

Abstract

The aim of the present study was to investigate the effect of gradually increasing glucose concentrations (from 5.6 to 111 mmol L−1) on the fermentative H2 production with and without bioaugmentation. A stirred tank reactor (STR) was operated at 70 °C and inoculated with a hyperthermophilic mixed culture or a hyperthermophilic mixed culture bioaugmented with Thermotoga neapolitana. With both the unaugmented (control) and augmented cultures, the H2 production rate was improved when the initial glucose concentration was increased. In contrast, the highest H2 yield (1.68 mol H2 mol−1 glucose consumed) was obtained with the augmented culture at the lowest glucose concentration of 5.6 mmol L−1 and was 37.5% higher than that obtained with the unaugmented culture at the same feed glucose concentration. Overall, H2 production rates and yields were higher in the bioaugmented cultures than in the unaugmented cultures whatever the glucose concentration. Quantitative polymerase chain reaction targeting T. neapolitana hydA gene and MiSeq sequencing proved that Thermotoga was not only present in the augmented cultures but also the most abundant at the highest glucose concentrations.

Original languageEnglish
Pages (from-to)17241-17249
Number of pages9
JournalInternational Journal of Hydrogen Energy
Volume45
Issue number35
DOIs
Publication statusPublished - 2020
Publication typeA1 Journal article-refereed

Keywords

  • Biohydrogen
  • Dark fermentation
  • Metabolic pathways
  • Microbial dynamics

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Fingerprint

Dive into the research topics of 'Enhancing thermophilic dark fermentative hydrogen production at high glucose concentrations via bioaugmentation with Thermotoga neapolitana'. Together they form a unique fingerprint.

Cite this