Exogenous GTP enhances the effects of sodium nitrite on cyclic GMP accumulation, vascular smooth muscle relaxation and platelet aggregation

K E Laustiola, P Vuorinen, I Pörsti, T Metsä-Ketelä, V Manninen, H Vapaatalo

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Exogenous guanosine triphosphate (GTP) (1-2 x 10(-4)M) resulted in increased concentrations of cyclic GMP both in endothelium denuded rat mesenteric artery (RMA) and in human ADP-stimulated platelets. Sodium nitrite (3.3 x 10(-4)M) relaxed precontracted RMA by 34%. When the arteries were preincubated with GTP (2 x 10(-4)M) sodium nitrite administration resulted in a significantly greater relaxation (58%) of the RMA with concomitant 2-fold increase in cGMP. Sodium nitrite (1 x 10(-4)M) had an inhibitory effect on ADP-induced platelet aggregation. Preincubation with GTP enhanced significantly the sodium nitrite-induced inhibition of ADP-induced platelet aggregation with a simultaneous 5-fold increase in cGMP. These results indicate that exogenous GTP enhances the sodium nitrite-induced stimulation of guanylate cyclase and thus enhances the effects of sodium nitrite on arterial smooth muscle and platelets.

Original languageEnglish
Pages (from-to)60-63
Number of pages4
JournalBasic Clin Pharmacol Toxicol.
Volume68
Issue number1
DOIs
Publication statusPublished - Jan 1991
Externally publishedYes
Publication typeA1 Journal article-refereed

Keywords

  • Animals
  • Cyclic GMP/metabolism
  • Drug Synergism
  • Guanosine Triphosphate/pharmacology
  • In Vitro Techniques
  • Male
  • Muscle, Smooth, Vascular/drug effects
  • Platelet Aggregation/drug effects
  • Platelet Aggregation Inhibitors/pharmacology
  • Rats
  • Rats, Inbred Strains
  • Sodium Nitrite/pharmacology
  • Vasodilation/drug effects

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