Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform

Stina Gröhn; Suvi Heinimäki; Kirsi Tamminen; Vesna Blazevic

doi:10.1007/s00705-020-04847-5

Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform

Stina Gröhn, Suvi Heinimäki, Kirsi Tamminen, Vesna Blazevic

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Recombinant protein technology enables the engineering of modern vaccines composed of a carrier protein displaying poorly immunogenic heterologous antigens. One promising carrier is based on the rotavirus inner-capsid VP6 protein. We explored different VP6 insertion sites for the presentation of two peptides (23 and 140 amino acids) derived from the M2 and HA genes of influenza A virus. Both termini and three surface loops of VP6 were successfully exploited as genetic fusion sites, as demonstrated by the expression of the fusion proteins. However, further studies are needed to assess the morphology and immunogenicity of these constructs.

Original language	English
Pages (from-to)	213–217
Journal	ARCHIVES OF VIROLOGY
Volume	166
Early online date	16 Oct 2020
DOIs	https://doi.org/10.1007/s00705-020-04847-5
Publication status	Published - 2021
Publication type	A1 Journal article-refereed

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00705-020-04847-5Licence: CC BY

2020_Article_-1Final published version, 1.65 MBLicence: CC BY

http://urn.fi/URN:NBN:fi:tuni-202012118753Licence: CC BY

Cite this

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abstract = "Recombinant protein technology enables the engineering of modern vaccines composed of a carrier protein displaying poorly immunogenic heterologous antigens. One promising carrier is based on the rotavirus inner-capsid VP6 protein. We explored different VP6 insertion sites for the presentation of two peptides (23 and 140 amino acids) derived from the M2 and HA genes of influenza A virus. Both termini and three surface loops of VP6 were successfully exploited as genetic fusion sites, as demonstrated by the expression of the fusion proteins. However, further studies are needed to assess the morphology and immunogenicity of these constructs.",

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AU - Blazevic, Vesna

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Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform

Abstract

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