Fast and Environmentally Friendly Quantitative Analysis of Active Agents in Anti-Diabetic Tablets by an Alternative Laser-Induced Breakdown Spectroscopy (LIBS) Method and Comparison to a Validated Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) Method

Victor Ulises Contreras, Marco A. Meneses-Nava, Nancy Ornelas-Soto, Oracio Barbosa-Garcia, Pedro L. Lopez-de-Alba, Jose L. Maldonado, Gabriel Ramos-Ortiz, Francisco J. Acevedo-Aguilar, Leticia Lopez-Martinez

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4 Citations (Scopus)

Abstract

Laser-induced breakdown spectroscopy (LIBS) is evaluated as a potential analytic technique for rapid screening and quality control of anti-diabetic tablets. This paper proposes a simple LIBS-based method for the quantitative analysis of two active pharmaceutical ingredients (APIs): metformin (Met) and glybenclamide (Gly). In order to quantify both APIs, chlorine (Cl) concentration was estimated by employing the Cl/Br optical emission ratio, where Br was introduced as internal standard. Calibration curves were prepared, achieving linearity higher than 99%. On the other hand, for comparison to the proposed method, an isocratic reversed-phase high-performance liquid chromatography (RP-HPLC) method was also developed for quantitative determination of the same analytes by ultraviolet (UV) detection. The chromatographic separation was achieved on a Phenomenex Hypersil C18, 250 mm x 4.6 mm, 5 mu m column. The mobile phase was K2HPO4/H3PO4-CH3OH and flow rate was 1.0 mL min(-1). The method is linear over a range of 10-60 mu g mL(-1) for Gly and 5-30 mu g mL(-1) for Met and the correlation coefficients were >= 0.99. Recoveries were found to be in the range of 95-101%. Furthermore, four different commercial brands of each active agent were evaluated by both proposed LIBS and chromatographic methods and results were compared with each other. The comparison was satisfactorily validated by analysis of variance (ANOVA).

Original languageEnglish
Pages (from-to)1294-1301
Number of pages8
JournalApplied Spectroscopy
Volume66
Issue number11
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes
Publication typeA1 Journal article-refereed

Keywords

  • Pharmaceuticals
  • API
  • Metformin
  • Glybenclamide
  • Laser-induced breakdown spectroscopy
  • LIBS
  • Spectroscopy
  • HUMAN PLASMA
  • METFORMIN
  • GLYBURIDE
  • MICRONUTRIENTS
  • SPECTROMETRY
  • MANAGEMENT
  • PRODUCTS
  • FLUORINE
  • CHLORINE
  • ASSAY

Publication forum classification

  • Publication forum level 1

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