Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules

Veikko Linko; Jenni Leppiniemi; Boxuan Shen; Einari Niskanen; Vesa P. Hytönen; J. Jussi Toppari

doi:10.1039/c1nr10518c

Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules

Veikko Linko, Jenni Leppiniemi, Boxuan Shen, Einari Niskanen, Vesa P. Hytönen, J. Jussi Toppari

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Abstract

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

Original language	English
Pages (from-to)	3788-3792
Number of pages	5
Journal	Nanoscale
Volume	3
Issue number	9
DOIs	https://doi.org/10.1039/c1nr10518c
Publication status	Published - 2011
Publication type	A1 Journal article-refereed

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title = "Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules",

abstract = "We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.",

author = "Veikko Linko and Jenni Leppiniemi and Boxuan Shen and Einari Niskanen and Hyt{\"o}nen, {Vesa P.} and Toppari, {J. Jussi}",

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T1 - Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules

AU - Linko, Veikko

AU - Leppiniemi, Jenni

AU - Shen, Boxuan

AU - Niskanen, Einari

AU - Hytönen, Vesa P.

AU - Toppari, J. Jussi

PY - 2011

Y1 - 2011

N2 - We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

AB - We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

U2 - 10.1039/c1nr10518c

DO - 10.1039/c1nr10518c

M3 - Article

AN - SCOPUS:80052585297

VL - 3

SP - 3788

EP - 3792

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 9

ER -

Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules

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