Heat Shock Protein 27 Is Involved in the Bioactive Glass Induced Osteogenic Response of Human Mesenchymal Stem Cells

Laura Hyväri, Sari Vanhatupa, Miina Ojansivu, Minna Kelloniemi, Toni Karri Pakarinen, Leena Hupa, Susanna Miettinen

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3 Citations (Scopus)
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Abstract

Bioactive glass (BaG) materials are increasingly used in clinics, but their regulatory mechanisms on osteogenic differentiation remain understudied. In this study, we elucidated the currently unknown role of the p38 MAPK downstream target heat shock protein 27 (HSP27), in the osteogenic commitment of human mesenchymal stem cells (hMSCs), derived from adipose tissue (hASCs) and bone marrow (hBMSCs). Osteogenesis was induced with ionic extract of an experimental BaG in osteogenic medium (OM). Our results showed that BaG OM induced fast osteogenesis of hASCs and hBMSCs, demonstrated by enhanced alkaline phosphatase (ALP) activity, production of extracellular matrix protein collagen type I, and matrix mineralization. BaG OM stimulated early and transient activation of p38/HSP27 signaling by phosphorylation in hMSCs. Inhibition of HSP27 phosphorylation with SB202190 reduced the ALP activity, mineralization, and collagen type I production induced by BaG OM. Furthermore, the reduced pHSP27 protein by SB202190 corresponded to a reduced F-actin intensity of hMSCs. The phosphorylation of HSP27 allowed its co-localization with the cytoskeleton. In terminally differentiated cells, however, pHSP27 was found diffusely in the cytoplasm. This study provides the first evidence that HSP27 is involved in hMSC osteogenesis induced with the ionic dissolution products of BaG. Our results indicate that HSP27 phosphorylation plays a role in the osteogenic commitment of hMSCs, possibly through the interaction with the cytoskeleton.

Original languageEnglish
Article number224
Number of pages18
JournalCells
Volume12
Issue number2
DOIs
Publication statusPublished - Jan 2023
Publication typeA1 Journal article-refereed

Funding

This work was supported by the Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital, Academy of Finland (Grant No. 326588, 312413 and 336666 to S.M.), Finnish Cultural Foundation (Grant No. 00190350, 00220359 to L.H.(Laura Hyväri)), The Finnish Concordia Fund (Grant No. 20200028 to L.H.(Laura Hyväri)), Alfred Kordelin Foundation (Grant No. 200198 to L.H.(Laura Hyväri)), City of Tampere science grant committee (L.H.(Laura Hyväri)), and the Tampere University Graduate School for Medicine and Life Sciences (L.H.(Laura Hyväri)).

Keywords

  • bioactive glass
  • mesenchymal stem cells
  • osteogenesis
  • p38/HSP27 signaling
  • phosphorylation

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology

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