Human intestinal organoid models for celiac disease research

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

Abstract

Celiac disease pathogenesis, in addition to immune cell component, encompasses pathogenic events also in the duodenal epithelium. In celiac disease patients, exposure to dietary gluten induces drastic changes in epithelial differentiation and elicit cellular response to inflammatory cytokines. The autoantigen in celiac disease, transglutaminase 2 (TG2) enzyme, has been also suggested to play its pathogenic gliadin deamidation event in the intestinal epithelium. Therefore in vitro epithelial cell-line models have been exploited in the past to study these pathogenic mechanisms, but they are hampered by their simplistic nature lacking proper cell-type composition and intestinal environ. Moreover, these cell models harbor many cancer-related mutations in tumor suppressor genes making them unsuitable for studying cell differentiation. Intestinal organoids provide a near-native epithelial cell model to study pathogenic agents and mechanisms related to celiac disease. Here we describe protocols to initiate and maintain celiac patient-derived organoid cultures and how to grow them in alternative ways allowing their exploitation in different kind of experiments.

Original languageEnglish
Title of host publicationCeliac Disease
EditorsAinara Castellanos-Rubio, Lorenzo Galluzzi
PublisherAcademic Press
Chapter14
Pages173-193
Number of pages21
ISBN (Print)978-0-443-19202-9
DOIs
Publication statusPublished - 2023
Publication typeA3 Book chapter

Publication series

NameMethods in Cell Biology
Volume179
ISSN (Print)0091-679X

Keywords

  • Celiac disease
  • Enterovirus infection
  • Epithelial differentiation
  • Intestinal organoids

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Cell Biology

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